Proteins and Peptides - BL-CYT-233: Recombinant Human Bone Morphogenetic Protein-7 (BMP-7)

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BL-CYT-233: Recombinant Human Bone Morphogenetic Protein-7 (BMP-7)

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Quantity:	2µg, 10µg, 1mg

Product Description Background : BMP-7 (osteogenic protein 1) belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. Description : Recombinant Human BMP-7 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 139 amino acids and having a molecular mass of 15679.97 Dalton. BMP-7 is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5. Solubility: It is recommended to reconstitute the lyophilized BMP-7 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Stability: Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below –18° C. Upon reconstitution BMP-7 should be stored at 4° C between 2-7 days and for future use below –18° C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Thr-Gly-Ser-Lys. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Applications: 1.Molecular standard (Western, ELISA) in studying secreted BMP-7 2.Preparing antibodies for BMP-7 monomer. 3.Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE. Endotoxin: Less than 0.1 ng/µg (IEU/µg) of Bone Morphogenetic Protein-7.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm 2. Analysis by RP-HPLC, using a standard solution of BMP-7 as a Reference Standard. Usage: This material is offered by BIOLOGO- for research, laboratory or further evaluation purposes. Gene: Name:BMP7 Synonyms:OP1 Protein synonyms/aliases: Bone morphogenetic protein 7 precursor (BMP-7) (Osteogenic protein 1) (OP-1). Protein Family: Belongs to the TGF-beta family. Domains found: • Transforming growth factor beta (TGFb), N-terminal • Transforming growth factor beta

References:
1. Mechanical testing of recombinant human bone morphogenetic protein-7 regenerated bone in sheep mandibles. Proc Inst Mech Eng [H] 2004;218(6):381-8
2. Sinus floor augmentation with simultaneous placement of dental implants in the presence of platelet-rich plasma or recombinant human bone morphogenetic protein-7. Clin Oral Implants Res 2004 Dec;15(6):716-23
3. Histological assessment of bioengineered new bone in repairing osteoperiosteal mandibular defects in sheep using recombinant human bone morphogenetic protein-7. Br J Oral Maxillofac Surg 2004 Oct;42(5):410-8
4. Bone morphogenetic protein 7: a novel treatment for chronic renal and bone disease. Curr Opin Nephrol Hypertens 2004 Jul;13(4):417-22
5. Bone morphogenetic protein-7 from serum of pregnant mice is available to the fetus through placental transfer during early stages of development. Nephron Exp Nephrol 2004;97(1):e26-32
6. Are recombinant human bone morphogenetic protein-7 and tobramycin compatible? An experiment in rats. J Orthop Trauma 2004 Apr;18(4):225-32