Research - VIP002-050: VIP, Vasoactive Intestinal Peptide

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VIP002-050: VIP, Vasoactive Intestinal Peptide

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Price: 480 € / 50 µl

Polyclonal Antibodies

Origin:	Guinea pig

Isotype:	Ig

Clone:	polyclonal

Format:	lyophil.

Quantity:	50 µl

Reconstitution:	Reconstitute with distilled water or PBS, mix only after 10 min, further dilution should be made with PBS or TBS containing 1% cristallin BSA and 0.09% sodium azide.

Working concentration:	1:1000 - 1:2000

Presentation:	Lyophilized original serum

Immunogen:	Synthetic human VIP (Peninsula)

Specificity:	VIP, Vasoactive Intestinal Polypeptide, antibody blocking with small amounts of VIP, but not with PHI

Species Specificity:	Human, rat, sheep and other vertebrates

General Information:	Vasoactive intestinal peptide (VIP) is a 28 aa neuropeptide hormone. Its sequence is identical in man, ox, pig and rat. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumours secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhoea, hypokalemia and achlorhydria syndrome. VIP shows high sequence homology with peptide histidine-methionine (PHM) in humans and peptide histidine-serine (PHI) in pig or rat and is exprimed in the same precursor.

Positive control:	Rat ileum (preferably Stefanini-fixed)

Pre-treatment:	Pre-treatment of paraffin material is not required, but enhanced staining may occur after application of high temperature unmasking (method not established).

Applications:	IHC(C, P)

Incubation time:	60 min at RT or 18 hr at 4°C

Secondary reagents:	We recommend the use of a specific anti guinea pig IgG conjugate e.g. goat-anti-guinea pig IgG:Biotin (VECTOR Art. no. BA-7000).

Storage:	2-8°C

References:
1. Messel T, et al. (1992) Extrinsic control of the release of galanin and VIP from intrinsic nerves isolated, perfused porcine ileum. Reg. Pept. 38;279-198.

2. Luts A., et al. (1993) Peptide containing nerve fibers in human airways: Distribution and coexistence pattern. Arch. Allergy Immunol. 101; 52-60.

3. Lopez-Figueroa M.O., et al. (1996) Presence of nitric oxide synthase in the sheep pineal gland: An experimental immunohistochemical study. Neuroendocrinology 63; 384-392.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.