Research - VEGC02-200: VEGF-C, rat (human)

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VEGC02-200: VEGF-C, rat (human)

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Price: 400 € / 200 µg

Polyclonal Antibodies

Origin:	Rabbit

Isotype:	IgG

Clone:	polyclonal

Format:	lyophilisate

Quantity:	200 µg

Concentration:	100 µg

Working concentration:	ELISA >2.5 µg/ml, Immunoprecipitation 1-10 µg/ml lysate or reaction volume, IHC not tested

Presentation:	Protein A purified IgG lyophilised from PBS pH 7.4 without preservative

Immunogen:	Recombinant rat delta-N-delta-C-VEGF-C (native protein)

Specificity:	Rat VEGF-C, no reactivity with VEGF-A and -D

Species Specificity:	Rat, cross-reaction with mouse and human

General Information:	VEGFs (Vascular Endothelial Growth Factors) are a group of homodimer glycoproteins. The subunits are linked by disulfide bonds. (MW reduced form 18-24 kDa). VEGFs are involved in angiogenesis during embryogenesis and in tumour progression and generation of metastasis. They possess mitogenic characteristics and specifically increase the permeability of endothelia in blood vessels.

Special properties:	The polyclonal antibody against VEGF-C is well suited to detect rat, human and mouse VEGF-C in body fluids, tissues and culture media.

Positive control:	Metastasing tumours of different origin

Applications:	ELISA, IP, IHC not tested

Secondary reagents:	As secondary reagents we recommend the VECTASTAIN ABC Elite Kit Rabbit IgG (Ref. PK-6101) or ImmPRESS anti-Rabbit IgG (Ref. MP-7401)

Storage:	-20°C

References:
1. Fitz LJ, Morris JC, Towler P, Long A, Burgess P, Greco R, Wang J, Gassaway R, Nickbarg E, Kovacic S, Ciarletta A, Giannotti J, Finnerty H, Zollner R, Beier DR, Leak LV, Turner KJ, Wood CR. Characterization of murine Flt4 ligand/VEGF-C. Oncogene. 1997 Jul 31;15(5):613-8.

2. Gu W, Brannstrom T, Jiang W, Bergh A, Wester P. Vascular endothelial growth factor-A and -C protein up-regulation and early angiogenesis in a rat photothrombotic ring stroke model with spontaneous reperfusion. Acta Neuropathol (Berl). 2001 Sep;102(3):216-26.

3. Tille JC, Wang X, Lipson KE, McMahon G, Ferrara N, Zhu Z, Hicklin DJ, Sleeman JP, Eriksson U, Alitalo K, Pepper MS. Vascular endothelial growth factor (VEGF) receptor-2 signaling mediates VEGF-C(deltaNdeltaC)- and VEGF-A-induced angiogenesis in vitro. Exp Cell Res. 2003 May 1;285(2):286-98.

4. Krishnan J, Kirkin V, Steffen A, Hegen M, Weih D, Tomarev S, Wilting J,Sleeman JP. Differential in vivo and in vitro expression of vascular endothelial growth factor (VEGF)-C and VEGF-D in tumors and its relationship to lymphatic metastasis in immunocompetent rats. Cancer Res. 2003 Feb 1;63(3):713-22.

5. Weich HA, Bando H, Brokelmann M, Baumann P, Toi M, Barleon B, Alitalo K, Sipos B, Sleeman J. Quantification of vascular endothelial growth factor-C (VEGF-C) by a novel ELISA. J Immunol Methods. 2004 Feb 15;285(2):145-55.

Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.