Research - TOP2001: Topoisomerase II (TOP2A)

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TOP2001: Topoisomerase II (TOP2A)

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Price: 370 € / 0.5 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	1E2

Format:	liquid conc.

Quantity:	0.5 ml

Concentration:	100 µg/ml

Working concentration:	1:20-1:100

Presentation:	Protein A purified IgG in PBS, BSA, NaN3

Immunogen:	Recombinant protein according to aa sequence 1435-1532 of human TOP2A

Specificity:	Topoisomerase II alpha (TOP2A), human

Species Specificity:	Human

General Information:	Topoisomerase II alpha plays an important role in the synthesis and transcription of DNA as well as in segregation of chromosomes during mitosis. The enzyme is thought to be a sensitive and specific marker for the late S-, G2 and M-phase of mitosis. TOP2A is also involved in s.c. multidrug resistance of tumour cells.

Positive control:	Tonsil

Pre-treatment:	Pre-treatment of formaldehyde-fixed tissue sections at 96-100°C in citrate buffer pH 6.0.

Applications:	IHC (C,P)

Incubation time:	60 min at RT or 18 hr at 2-8°C

Secondary reagents:	Recommended secondary systems in IHC are our Universal Staining Kits Ref. AE005 or DA005 or the VECTASTAIN Elite ABC System Mouse IgG (Ref. PK-6102). The latter is also suitable for Western Blotting or other techniques.

Storage:	2-8°C

References:
1. Roula Albadinea, Wenle Wanga, Noel A. Brownleea et al. (2009) Topoisomerase II ? Status in Renal Medullary Carcinoma: Immuno-Expression and Gene Copy Alterations of a Potential Target of Therapy 182;735-740

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!