Research - PDP001-0.5: Podoplanin (PDPN, M2A Antigen) (D2-40)

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PDP001-0.5: Podoplanin (PDPN, M2A Antigen) (D2-40)

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Price: 375 € / 0.5 ml

Monoclonal Antibody

Origin:	Mouse

Isotype:	IgG1

Clone:	D2-40

Format:	liquid conc.

Quantity:	0.5 ml

Working concentration:	1:30-1:100

Presentation:	Affinity purified antibody in PBS, BSA, NaN3

Immunogen:	M2A Antigen, Podoplanin

Specificity:	Podoplanin, M2A Antigen of lymphatic endothelia

Species Specificity:	Human, other species not tested

General Information:	Podoplanin is a highly glycosylated glycoprotein (162 AS, 36 kDa) that is predominantly expressed on lymphatic endothelia. Therefore antibodies against podoplanin are often used as a marker for lymphatic vessels. Podoplanin is a protein that is also found on podocytes of the kidney glomeruli (therefore the name podoplanin) and in fetal testis. Since a while podoplanin is considered as a good marker for the malign pleuramesothelioma and is used for differential diagnosis of this disease (Ordonèz, 2005). Normally podoplanin occurs in lymphatic vessels, placenta, lung, and skeletal muscles. Appearance in testicular germ cells, lymphangioma and lymphangiosarcoma is noticeable. In haemangioma und other diseases of the vessels overexpression of the glycoprotein is not found.

Special properties:	This antibody is well suited for the detection of podoplanin in histological sections and cells.

Positive control:	Lymph nodes, Germ cell tumors

Pre-treatment:	The best pre-treatment of paraffin sections has not been validated at our lab. Meanwhile we suggest the usage of routine heat induced antigen retrieval although some laboratory results were positive without any unmasking.

Applications:	IHC (C,P)

Secondary reagents:	Anti-mouse IgG:Biotin conjugate (code no. ZU102) in combination with Streptavidin-HRPO (code no. ZU054) and a suitable substrate e.g. DAB (Ref. DA002) or AEC (Ref AE002).

Storage:	2-8°C

UniProt Accession No.: Q86YL7 (PDPN_HUMAN)

References:
1. Hiroaki Yokomori Masaya Oda, Fumihiko Kaneko, Shigeyuki Kawachi, Minoru Tanabe, Kazunori Yoshimura, Yuko Kitagawa, and Toshifumi Hibi Lymphatic marker podoplanin/D2-40 in human advanced cirrhotic liver- Re-evaluations of microlymphatic abnormalities Gastroenterology 2010, 10:131

2. Annette M. Müller, Folker E. Franke, Klaus-Michael Müller (2006) D2-40: A Reliable Marker in the Diagnosis of Pleural Mesothelioma Pathobiology 73:50-54

3. Yu H, Pinkus GS, Hornick JL. Diffuse membranous immunoreactivity for podoplanin (D2-40) distinguishes primary and metastatic seminomas from other germ cell tumors and metastatic neoplasms. Am J Clin Pathol. 2007 Nov;128(5):767-75.

4. Qingmei Xie, Lugen Chen, Kai Fu, Josephine Harter, Ken H Young, Jaya Sunkara, Deborah Novak, Esperanza Villanueva-Siles, and Howard Ratech (2008) Podoplanin (D2-40): A New Immunohistochemical Marker for Reactive Follicular Dendritic Cells and Follicular Dendritic Cell Sarcomas Int J Clin Exp Pathol. 1(3): 276–284.

5. Nelson G. Ordóñez (2005) D2-40 and podoplanin are highly specific and sensitive immunohistochemical markers of epithelioid malignant mesothelioma Human Pathol. 36(4) 372-380

Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

The preservative sodium azide has been added in very small quantities. Nevertheless, it may be harmful to health and the reagent should be used only by trained personnel. For waste disposal, please refer to national regulations.