Research - NC100: N-CAM, Neuronal Cell Adhesion Molecule

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NC100: N-CAM, Neuronal Cell Adhesion Molecule

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Price: 310 € / 1 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	RNL-1

Format:	liquid conc.

Quantity:	1 ml

Working concentration:	1:10-1:25

Presentation:	Cell culture supernatant containing 0.09% sodium azide as preservative

Immunogen:	NCI-H82 cell preparation

Specificity:	Extracellular domain of the N-CAM molecule (200, 100 and 25-27 kDa proteins of NCI-H82 SCLC cell line in western blot)

Species Specificity:	Human

General Information:	N-CAM is an antigen, that is characteristic of cells of neuroectodermal origin. In the blood it is mainly found on NK-cells (CD56). It is a heavily glycosylated trans-membrane protein. On the 3. International Workshop for Lung tumours and Differentiation antigens (Zürich 1993) N-CAM was assigned to cluster 1 antigens of lung carcinoma (typical for SCLC).

Special properties:	Antibody RNL-1 shows heavy N-CAM expression on cells of the small cell lung carcinoma (SCLC), neuroblastoma, nerves, Leydig-cells, thyroid epithelia and parafollikular cells, pancreas islet cells, adrenal cortex and medulla, and hypophysis. Little differentiated squamous lung tumours may by RNL-1 positive, while adenocarcinoma of the lung are negative. The antibody may be used for differentiating the types of lung tumours.

Positive control:	Pancreas islets or adrenal medulla

Pre-treatment:

Applications:	IHC(C), suitability for paraffin sections not established. Glutaraldehyde fixation for EM has been used successfully (Broers et al. 1991).

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Broers V.J.L., Mijnheere E.P., Rot M.K., Schaart G. Sijlmans A., Boerman O.C., and Raaemaekers F.C.S. (1991) Novel antigens charakteristic of neuroendocrine malignancies. Cancer 67,3; 619-633.

2. Boerman O.C., Mijnheere E.P., Broers J.L.V., Vooijs G.P., and Ramaekers F.C.S. (1991) Biodistribution of a monoclonal antibody (RNL-1) against the neural cell adhesion molecule (NCAM) in athymic mice bearing human small-cell lung-cancer xenografts. Int. J. Cancer 48; 457-462.

3. Stahel R.A., Gilks W.R., Schenker Th., and Gerardy-Schahn R. (1995) NK3.1 Third International Workshop on Lung Tumour and Differentiation Antigens: summary and report on NCAM studies. In: Leucocyte Typing V. Schlossman S.F. et al. eds. Oxford University Press, Oxford, N.Y. Tokyo.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!