Research - KRN7000-1: α-Galactosylceramide (α-GalCer)

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KRN7000-1: α-Galactosylceramide (α-GalCer)

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Price: 320 € / 1 mg

Ceramide

Molecular weight:	858.34

Origin:	synthetic

Format:	Powder

Quantity:	1 mg

Reconstitution:	In vitro assay：KRN7000 should be dissolved by DMSO at the concentration of 1mg/ml with heating at 60-80℃ for tens of seconds. The solution of 1mg/ml in DMSO can be diluted by PBS.

Structure of α-GalCer (klick to enlarge)

Presentation:	White to off-white powder

General Information:	KRN7000, a marine sponge glycolipid with an α-galactosylceramide (α-GalCer) structure, was found to exhibit potent antitumor activity in a variety of experimental and spontaneous tumor metastasis models. The antitumor effects of α-GalCer were first shown to be attributable to NKT cell activation and the antigen presentation function of dendritic cells. α-GalCer was then identified as a ligand presented by CD1d to the invariant Vα14 T cell antigen receptor of mouse NK1.1+αβTCR+ (NKT) cells, and the invariant Vα24JαQVβ11 TCR of human NKT cells. NKT cells have a range of characteristics that distinguish them from conventional T and NK cells. In particular, upon stimulation, NKT cells have a remarkable capacity to produce immunoregulatory cytokines, including IL-4, IL-10, IL-13, and IFN-γ, which can powerfully influence the nature of adaptive immune responses. NKT cells also express a wide variety of cell death-inducing effector molecules and have been demonstrated to kill tumor target cells in vitro. NKT cells assist in the prevention of autoimmune diseases, such as diabetes (an immune attack on the pancreas), multiple sclerosis (an immune attack on the nerves), inflammatory bowel diseases (IBD), and an immune-mediated disease of the intestine, which include Crohn's disease and ulcerative colitis.

Special properties:	Solubility KRN7000 is practically insoluble in water, methanol or ethanol, very slightly soluble in tetrahydrofuran, slightly soluble in pyridine, and practically insoluble in other organic solvents.

Storage:	2-8°C short term, -20°C long term

References:
Kohji Akimoto, Takenori Natori and Masahiro Morita (1993) Synthesis and stereochemistry of agelasphin-9b Tetrahedron Letters Volume 34, Issue 35, pp 5593-5596

Notes:
Licensed by Kyowa Hakko Kirin Co., Ltd.

Product use only for in vitro research!