Research - IL1A001: Interleukin-1 alpha (IL-1?)

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IL1A001: Interleukin-1 alpha (IL-1?)

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Price: 480 € / 50 µg

Polyclonal Antibodies

Origin:	Mouse

Isotype:	IgG2a

Clone:	polyclonal

Format:	liquid conc.

Quantity:	50 µg

Concentration:	1 mg/ml

Working concentration:	1/500-1/1000 Western Blot, 1/400 IHC (Paraffin)

Presentation:	Protein A purified IgG in PBS, pH 7.4, 0.03% sodium azide*

Immunogen:	Ni-NTA purified recombinant human IL-1 alpha, exprimed in E. coli strain BL21 (DE3)

Specificity:	Human Interleukin-1 alpha

Species Specificity:	Human

General Information:	Interleukin 1 alpha is exprimed as 31 kDa pro-molecule, which does not contain a signal sequence. By proteolytic cleaving the mature 17 kDa IL-1 alpha is generated. This peptide is released upon injury and apoptosis. IL-1 alpha stimulates the activity of osteoclasts in bone nad plays a role in the modulation of the extracellular calcium concentration. It activates T-cells, B-cells, and NK-cells. Endotoxins initiate the signal transduction of the pro-molecule and activate further pro-inflammatoric genes in the nucleus.

Positive control:	Spleen

Applications:	IHC (C,P), ELISA, WB

Incubation time:	IHC(P) 60 min at RT or 2-8°C over night

Secondary reagents:	Biotinylated anti-mouse IgG (Art. No. ZU102) in combination with Streptavidin:HRPO (Art. No. ZU054) or Streptavidin:AP (Art. No. ZU051) and a suitable substrate.

Storage:	2-8°C for 1 month, aliquotes at -20° for

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!