Research - HNP103: Alpha-DEFENSIN (1-3), HNP1-3

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HNP103: Alpha-DEFENSIN (1-3), HNP1-3

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Price: 480 € / 100 µg

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	DEF-3

Format:	lyophilisate

Quantity:	100 µg

Concentration:	200 µg/ml IgG after reconstitution with 0,5 ml distilled water

Working concentration:	0.2 µg/ml IHC(C), 0.5 µg/ml IHC(P)

Presentation:	Affinity purified IgG, lyophilized from PBS, BSA, and sodium azide**, reconstitute with 0.5 ml sterile water to get a stock solution of 200 µg/ml.

Immunogen:	Native Defensins

Specificity:	Human Neutrophil Peptides (HNP1-3) syn. alpha-Defensins 1-3, reactivity with HNP-4 not tested

Species Specificity:	Human, cross-reactivity with rhesus monkey and primates expected

General Information:	Human defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated microorganisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4) are known.

Special properties:	The monoclonal antibody DEF-3 reacts with human alpha-defensins (HNP1-3), which are synthesized and stored by neutrophilic granulocytes.

Positive control:	Infected skin or oral mucosa

Pre-treatment:	Formaldehyde-fixed tissue requires antigen unmasking preferred methods are 0.1% Pronase, Proteinase K or Trypsin.

Applications:	IHC (C,P), FACS

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	-20'°C'

References:
1. Ganz T. et al. (1985) J. Clin. Invest., 76; 1427 ff

2. Ganz T., et al. (1992) Med. Microbiol. Immunol. 181; 99ff

3. Mizukawa N ; Sugiyama K ; Fukunaga J ; Ueno T ; Mishima K ; Takagi S ; Sugahara T (1998) Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma patients. Anticancer Res 18(6B):4645-4649.

4. Linzmeier R., Ho C.H., Hoang B.V. , and Ganz T. (1999) A 450 kb contig of defensin genes on human chromosome 8p23. Gene 233; 205-211.

5. Mikuzawa N. Sugiyama K., Kamio M., Yamachika E., Ueno T., Fukunaga J., Takagi S., Sugahara T. (2000) Immunohistochemical staining of human alpha-defensin-1 (HNP-1) in submandibular glands of patients with oral carcinomas. Anticancer Res. 20(2B); 1125-1127.

Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!