Research - GLU001: Glucagon

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GLU001: Glucagon

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Price: 280 € / 0.25 ml

Polyclonal Antibodies

Origin:	Rabbit

Isotype:	Ig

Clone:	polyclonal

Format:	liquid conc.

Quantity:	0.25 ml

Working concentration:	1:50

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Immunogen:	Porcine pancreatic Glucagon-BSA conjugate

Specificity:	Glucagon

Species Specificity:	Human, rat, pig

General Information:	Glucagon is a polypeptide which is produced by s.c. A-cells of the pancreatic islets. It is the counterpart of insulin in the regulation of blood sugar levels. Except of the pancreas, glucagon exists also in endocrine cells of the stomach and in neurons of the brain. Frequently it has been found in pancreatic tumours and in rectal carcinoids, which can be responsible for glucagonoma syndromes.

Special properties:	The antibody is well suited for the detection of glucagon in tissue sections of brain, pancreas and stomach.

Positive control:	Pancreas

Pre-treatment:	No protease pre-treatment required. Improvement may be achieved by HIER.

Applications:	IHC (C,P)

Incubation time:	IHC(P) 60 min at RT or 2-8°C over night

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005), for higher sensitivities VECTASTAIN Elite ABC (Art. No. PK-6101) or ABC AP (Art. No. AK-5001) systems are applicable.

Storage:	2-8°C

References:
1. Böttcher G, Ahrén B, Lundquist I, Sundler F. (1989) Peptide YY: intrapancreatic localization and effects on insulin and glucagon secretion in the mouse. Pancreas. 4(3):282-8.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!