Research - ERG001-1.0: ERG (TMPRSS2-ERG)

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ERG001-1.0: ERG (TMPRSS2-ERG)

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Price: 720 € / 1 ml

Monoclonal Antibody

Origin:	Rabbit

Isotype:	IgG

Clone:	EPR3864

Format:	liquid conc.

Quantity:	1 ml

Working concentration:	1/100

Presentation:	Protein A purified antibody in PBS, BSA, sodium azide (0,09%)

Immunogen:	C-terminal synthetic peptide of human ERG

Specificity:	Human and murine ERG, detects also Fli-1

Species Specificity:	Human, mouse, rat

General Information:	Transcriptional Regulator ERG belongs to the ETS family that is involved in the regulation of a wide variety of cellular and developmental processes. This family of transcription factors contains approximately 30 members that share a highly conserved DNA-binding domain (ETS domain) and differs from each other in various domains (such as absence or presence of the Pointed/SAM domain) and are thus distinguished in sub-families. ERG belongs to the Erg/Fli-1 sub-family. It has been widely studied for its involvement in human cancers, TMPRSS2-ERG fusion, which occurs on account of translocations and interstitial deletions, is implicated in aggressive forms of prostate cancer. ERG is linked to normal processes such as mesoderm formation and is found to form functional complexes with Jun/Fos, with the resulting ternary complexes regulating expression of genes such as metalloprotease-1 (MMP-1) and MMP-3. It may also participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. ERG is expressed in virtually all vascular tumors and approximately 50% cases of prostate adenocarcinomas.

Positive control:	ERG-positive prostate carcinoma

Pre-treatment:	Pre-treatment of dewaxed slices with heat induced epitope retrieval method is recommended. Use TRIS/Borate/EDTA buffer, Citrate Buffer (code no. DE000) or Unmasking Solution G (code no. DE007) .

Applications:	IHC(C,P), ICC, WB

Secondary reagents:	Anti-rabbit IgG:Biotin conjugate (code no. ZU101) in conjunction with Streptavidin-HRPO (code no. ZU054) or VECTOR ImmPress rabbit IgG Kit (code no. MP-7401).

Storage:	2-8°C

UniProt Accession No.: B2Y833 (B2Y833_HUMAN)

References:
1. Park K, Tomlins SA, Mudaliar KM, Chiu YL, Esgueva R, Mehra R, Suleman K, Varambally S, Brenner JC, MacDonald T, Srivastava A, Tewari AK, Sathyanarayana U, Nagy D, Pestano G, Kunju LP, Demichelis F, Chinnaiyan AM, Rubin MA. Antibody-based detection of ERG rearrangement-positive prostate cancer. Neoplasia. 2010 Jul;12(7):590-8

2. Chaux,A., Albadine,R., Toubaji,A., Hicks,J., Meeker,A., Platz,E.A et al. (2011) Immunohistochemistry for ERG expression as a surrogate for TMPRSS2-ERG fusion detection in prostatic adenocarcinomas, Am. J. Surg. Pathol. 35 (7), 1014-1020 PUBMED 21677539

3. Yaskiv,O., Zhang,X., Simmerman,K., Daly,T., He,H., Falzarano,S., Chen,L., Magi-Galluzzi,C. and Zhou,M. (2011) The utility of ERG/P63 double immunohistochemical staining in the diagnosis of limited cancer in prostate needle biopsies, Am. J. Surg. Pathol. 35 (7), 1062-1068 PUBMED 21623182

4. Miettinen,M., Wang,Z.F., Paetau,A., Tan,S.H., Dobi,A., Srivastava,S. and Sesterhenn,I. (2011) ERG transcription factor as an immunohistochemical marker for vascular endothelial tumors and prostatic carcinoma, Am. J. Surg. Pathol. 35 (3), 432-441 PUBMED 21317715

Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

The preservative sodium azide has been added in very small quantities. Nevertheless, it may be harmful to health and the reagent should be used only by trained personnel. For waste disposal, please refer to national regulations.