Research - CTN120-0.5: p120 Catenin (Catenin Delta 1)

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CTN120-0.5: p120 Catenin (Catenin Delta 1)

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Price: 380 € / 0.5 ml

Monoclonal antibody

Origin:	Mouse

Isotype:	IgG1

Clone:	MRQ-5

Format:	liquid conc.

Quantity:	0.5 ml

Working concentration:	1:50-1:200 (IHC)

Presentation:	Affinity purified antibody in PBS containing 0.2%BSA and 0.09% sodium azide

Immunogen:	Synthetic human p120 catenin protein

Specificity:	Catenin Deta 1; p120 Catenin

Species Specificity:	Human

General Information:	p120 Catenin binds E-Cadherin at a juxta-membrane site, while alpha-Catenin and beta-Catenin bind to the intracellular domain of E-Cadherin. The complex stabilizes tight junctions. A deficiency of E-Cadherin results in the intracytoplasmic accumulation of p120 Catenin. Lobular carcinoma of the breast shows intracytoplasmic accumulation of p120 Catenin while ductal carcinoma shows reduced membrane p120 Catenin without cytoplasmic accumulation. In gastric and colonic carcinoma, strong cytoplasmic p120 Catenin is associated with dyscohesive infiltrative morphology. The high expression of p120 may indicate poor prognosis in lung adenocarcinoma, bladder cancer, pancreatic cancer.

Positive control:	Lobular carcinoma of the breast, Melanoma

Pre-treatment:	Pretreatment of paraffin sections at high temperature is required. Suitable buffers are 0.01 M Citrate buffer (Art. No. DE000) or TRIS/EDTA buffer (pH 9; Art. No. DE006).

Applications:	IHC(C, P)

Secondary reagents:	Anti-mouse IgG conjugate, biotinylated (Art. No.ZU102) in combination with streptavidin:HRPO (Art. No. ZU054)

Storage:	2-8°C

References:
1. Reynolds AB, Herbert L, et al. p120, a novel substrate of protein tyrosine kinase receptors and of p60, is related to cadherin-binding factors β-catenin, plakoglobin, and armadillo. Oncogene 1992;7: 2439-2445.

2. Thoreson MA, Anastasiadis PZ, et al. Selective uncoupling of p120 from E-cadherin complexes. Mol Cell Biol. 1994;14: 8333-8342.

3. Sarrio D, Perez-Mies B, et al. Cytoplasmic localization of p120ctn and E-cadherin loss characterize lobular breast carcinoma from preinvasive to metastatic lesions. Oncogene. 2004 Apr 22;23(19):3272-83.

4. Dabbs DJ, Bhargava R, Chivukula M. The diagnostic utility of p120 catenin. Am J Surg. Pathol. 2007 31(3):427-37.

Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

The preservative sodium azide has been added in very small quantities. Nevertheless, it may be harmful to health and the reagent should be used only by trained personnel. For waste disposal, please refer to national regulations.