Research - CK118: Cytokeratin 18

Products:

AGE

BioPrime Antibodies

BioPrime Staining Reagents

Proteins and Peptides

VECTOR Reagents

Products> Research

CK118: Cytokeratin 18

Print page

place in shopping cart

Price: 285 € / 1 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	DA7

Format:	liquid conc.

Quantity:	1 ml

Concentration:	S/N

Working concentration:	1:5-1:20

Presentation:	Cell culture supernatant containing 0.09% sodium azide as preservative

Immunogen:	Human mamma carcinoma cell line PMC-42

Specificity:	45 kDa Cytokeratin

Species Specificity:	Human and other mammalians

General Information:	Cytokeratins are a group of water insoluble filament proteins, which are constituents of the cytoskeleton of epidermal cells and other epithelial cells. By gel electrophoretic analysis up to now 20 different cytokeratins have been characterized. They have been divided into basic and acid subfamilies and may also be distinguished by their molecular weights and their tissue distribution. The most frequently used nomenclature has been published by R. Moll et al. (1982).

Special properties:	This Cytokeratin 18 antibody reacts with most adenocarcinoma and basal cell carcinoma. It has been used used for the differentiation between adenocarcinoma and mesothelioma on the one hand side (positive reaction) and squamous epithelial carcinoma and non-epithelial tumours on the other side (CK18 negativ).

Positive control:	Mamma carcinoma or appendix

Pre-treatment:	Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid C or G (Art. No. DE000 and DE007)

Applications:	IHC (C,P)

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C or aliquots at -20°C

References:
1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff.

2. Lauerova L., et al. (1988) Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule Folia Biol. Praha 35(6); 373-382.

3. Voitesek B., et al. (1989) Monoclonal antibodies recognizing different epitopes of cytokeratin 18 molecule. Hybridoma 7; 495-504.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!