Research - CK1001: Cytokeratin 1

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CK1001: Cytokeratin 1

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Price: 300 € / 40 µg

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	A00814.01

Format:	liquid conc.

Quantity:	40 µg

Concentration:	1 mg/ml

Working concentration:	0.5-2 µg/ml

Presentation:	Purified antibody in 10 mM PBS pH 7.6, 0,02% NaN3

Immunogen:	Ni-NTA-purified sequence of recombinant human CK1, exprimed in E. coli strain BL21 (DE3)

Specificity:	Cytokeratin 1 (CK1, syn. KRT1) 644 AS, ca. 70 kDa

Species Specificity:	Human, other vertebrates not tested

General Information:	Cytokeratins are a group of water insoluble filament proteins, which are constituents of the cytoskeleton of epidermal cells and other epithelial cells. By gel electrophoretic analysis up to now 20 different cytokeratins have been characterized. They have been divided into basic and acid subfamilies and may also be distinguished by their molecular weights and their tissue distribution. The most frequently used nomenclature has been published by R. Moll et al. (1982).

Special properties:	CK1 is produced in the epidermis especially in Stratum spinosum and Stratum granulosum. Usually it occurs together with CK10 (KRT10). Mutations of the respective genes are associated with idiopathic ichthyosiform erythroderma.

Positive control:	Skin, liver

Pre-treatment:	Pre-treatment of formaldehyde-fixed tissue with proteinase E (Unmasking Fluid P, Art. No. DE110), citrate buffer (Art. No. DE000) or Unmasking Fluid G (Art. No. DE007).

Applications:	IHC(C,P), WB, ELISA

Incubation time:	60 min at RT or over night at 2-8°C

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff.

2. Lucas S.D., Ek B., Rask L., Rastad J., Akerstr;m G., and Juhlin C. (1997) Aberrantly expressed cytokeratin 1, a tumour-associated autoantigen in papillary thyroid carcinoma. Cancer 73: 171-177.

3. Ban. M., Yoneda K., and Kitajima Y. (1997) Differentiation of eccrine poroma cells to cytokeratin 1- and 10-expressing cells, the intermediate layer cells of eccrine sweat duct in the tumour cell nests. J. Cut. Pathol. 24: 246-248.

4. Hama Naoto (2005) Immunohistochemical Study of cytokeratin related protein expression in patients with trichilemmal tumors and squamous cell carcinoma of the skin. Dokkyo J. Med. Sci. 32: 7-12.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!