Research - CDK004-0.5: Cdk4 (Cyclin-dependent kinase 4)

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CDK004-0.5: Cdk4 (Cyclin-dependent kinase 4)

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Price: 300 € / 0.5 ml

Polyclonal Antibody

Origin:	Rabbit

Isotype:	Ig

Clone:	polyclonal

Format:	liquid conc.

Quantity:	0.5 ml

Concentration:	200 µg/ml

Working concentration:	2-20 µg/ml

Presentation:	Affinity purified antibody with PBS, 0.1% gelatine, and 0.09% sodium azide

Immunogen:	Peptide mapping at the C-terminus of Cdk4 of mouse origin.

Specificity:	CDK4 (Cyclin Dependent Kinase 4)

Species Specificity:	Mouse, Human, Rat

General Information:	Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (CDKs). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also regulate the cell cycle by binding to cyclin-Cdk complexes and modulate their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. Association with a D-type cyclin up regulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 down regulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK).

Special properties:	The antibody against CDK4 is well suited for the detection of the enzyme in G1-phase of different cell types (e.g.. Ramos-cells, HeLa cells, and K-562 cells

Positive control:	Ramos-cells, HeLa cells, NIH/3T3 cells, human skin

Pre-treatment:	Optimum pre-treatment of formalin-fixed tissue has not been established at BIOLOGO. Please try out the different methods available. Immunofluorescence labelling performs best when cells have been fixed with ice cold methanol.

Applications:	WB, IFA, ICC, IHC(P), ELISA, IP

Secondary reagents:	Anti-rabbit IgG:Biotin conjugate (code no. ZU101) in conjunction with Streptavidin-HRPO (code no. ZU054) or VECTOR ImmPress rabbit IgG Kit (code no. MP-7401).

Storage:	2-8°C

UniProt Accession No.: P30285 (CDK4_MOUSE)

References:
1. Villalonga, P., et al. 2000. [Lys61]N-Ras is able to induce full activation and nuclear accumulation of Cdk4 in NIH/3T3 cells. Oncogene 19: 690-699.

2. Sotillo, R., et al. 2001. Wide spectrum of tumors in knock-in mice carrying a Cdk4 protein insensitive to INK4 inhibitors. EMBO J. 20: 6637-6647.

3. Freeman, Scott N., Analysis of E2F1 target genes involved in cell cycle and apoptosis" (2007). Theses and Dissertations. Paper 2178.

Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

The preservative sodium azide is potentially hazardous and should be used by trained personal only. Disposal only in accorcance with local rules.