Research - CD54-1: CD54, ICAM-1

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CD54-1: CD54, ICAM-1

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Price: 380 € / 100 µg

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG2a

Clone:	1304

Format:	lyophil.

Quantity:	100 µg

Concentration:	0.1 mg lyophilized

Working concentration:	2 µg/ml (IHC-P)

Presentation:	Affinity purified, lyophilized antibody. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 10mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative.

Immunogen:	Cultivated human endothelial cells

Specificity:	Human CD54, the antibody detects an epitope in domain 1 of the 95 kDa transmembrane protein.

Species Specificity:	Human

General Information:	Intercellular adhesion molecule (ICAM) 1 or CD54 was initially identified with monoclonal antibody RR1/1 as a widely distributed, cytokine-inducible counter-receptor for lymphocyte-function-associated antigen (LFA) 1 (CD11a/CD18). Granulomas (macrophages/epitheloid cells) in inflamed colon from Crohn's disease and follicular dendritic cells in the tonsil from chronic tonsillitis are strongly positive for CD54 antigen. Also, CD54 is found on activated CD56+/CD3- NK cells.

Special properties:	Monoclonal antibody 1304 detects CD54 (ICAM-1) and is useful for the detection of activated endothelial cells and other activated haematopoietic cells. The antibody inhibits the binding of natural killer (NK) cells to target cells (K562).

Positive control:	Human Tonsil

Pre-treatment:	Formaldehyde-fixed tissue slices are preferably pre-treated with proteinase K.

Applications:	IHC (C,P)

Incubation time:	60 min at RT or 18 hr at 2-8°C

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Albelda S.M., and Buck C.A. (1990) Integrins and other cell adhesion molecules. FASEB J. 4; 2868-2880.

2. Freudenthal P.S., and Steinmann R.M. (1990) The distinct surface of human blood dendritic cells, as observed after an improved isolation method. PNAS 87; 7698-7702.

3. Klickstein L.B., and Springer T.A. (1995) CD54 (ICAM-1)cluster report. In Leucocyte Typing V: White Cell Differentiation Antigens. S. Schlossman et al. eds.Oxford University Press pp.1548-1550.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!