Research - BCL010: Bcl-10 Oncoprotein

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BCL010: Bcl-10 Oncoprotein

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Price: 410 € / 1 ml

Polyclonal Antibodies

Origin:	Rabbit

Isotype:	Ig

Clone:	polyclonal

Format:	liquid conc.

Quantity:	1 ml

Working concentration:	1:50-1:100

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Immunogen:	Bcl-10 aa sequence 216-233 coupled to KLH

Specificity:	Human Bcl-10 (32 kDa)

Species Specificity:	Human, mouse, rat

General Information:	Bcl-10 (syn. CIPER, CARMEN and CLAP) is a protein which is involved in apaoptosis by induction of Caspase 9. Bcl-10 overexpression induces JNK, p38-MAPK and NF-kB activation.

Special properties:	Mucosa Associated Lymphoid Tissue' (MALT)-B-Zell Lymphoma with 1;14-(p22;q32)-translocation are associated with the overexpression of Bcl-10 protein. Bcl-10 mutations are also observed in cases of follicular lymphoma and in diffuse large B-cell lymphoma. In normal tissues Bcl-10 is found in lymphoid organs and in the cytoplasma of mammary gland cells. In MALT lymphoma Bcl-10 usually is detected in cytoplasma as well as in the nuclei.

Positive control:	Raji Burtkitt lymphoma or THP-1 acute monocytic le

Applications:	C, P

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Willis TG, Jadayel DM, Du MQ, Peng H, Perry AR, Abdul-Rauf M, Price H, Karran L, Majekodunmi O, Wlodarska I, Pan L, Crook T, Hamoudi R, Isaacson PG, Dyer MJ. Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple tumor types. Cell. 1999 Jan 8;96(1):35-45.

2. Gill S, Broni J, Jefferies S, Osin P, Kovacs G, Maitland NJ, Eeles R,Edwards SM, Dyer MJ, Willis TG, Cooper CS. BCL10 is rarely mutated in human prostate carcinoma, small-cell lung cancer, head and neck tumours, renal carcinoma and sarcomas. MPT Collaborators, St George's Hospital Collaborators. Br J Cancer. 1999 Jul;80(10):1565-8.

3. Du MQ, Peng H, Liu H, Hamoudi RA, Diss TC, Willis TG, Ye H, Dogan A, Wotherspoon AC, Dyer MJ, Isaacson PG. BCL10 gene mutation in lymphoma. Blood. 2000 Jun 15;95(12):3885-90.

4. Ye H, Dogan A, Karran L, Willis TG, Chen L, Wlodarska I, Dyer MJ, Isaacson PG, Du MQ. BCL10 expression in normal and neoplastic lymphoid tissue. Nuclear localization in MALT lymphoma. Am J Pathol. 2000 Oct;157(4):1147-54.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!