Research - APO002-100: Apolipoprotein D

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APO002-100: Apolipoprotein D

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Price: 360 € / 100 µl

Monoclonal Antibodies

Origin:	Rabbit

Isotype:	IgG

Clone:	EPR2916

Format:	liquid conc.

Quantity:	100 µl

Working concentration:	1:50-1:100

Presentation:	Purified antibody in BPS containing BSA and 0,09% sodium azide

Immunogen:	Synthetic human Apolipoprotein D sequence

Specificity:	Human Apolipoprotein D

Species Specificity:	Human

General Information:	Apolipoprotein D (Apo D) is a 24 kDa glycoprotein, which is involved in lipid transport in the blood. It is also a progesteron-binding protein and forms complexes with lecithin. This leeds to the assumption that it plays a role in esterification of cholesterol. Apo D is present in normal cell especially in nervous tissue, apocrine glands in the skin, breast glands, pituitary, kidney, Corpus luteum, testis and Prostate. It is an early marker of prostate carcinoma and in the skin it is a relatively specific marker for the differentiation of dermatofibrosarcoma protuberans.

Special properties:	The antibody is well suited for the detection of Apo D in different tissues and tissue extracts. In Western Blots a band of 21-24 kDa is detected.

Positive control:	Testis (Leydig cells), nerve tissue

Pre-treatment:	Formaldehyde-fixed material should be pre-treated by heat induced unmasking methods. Good results have been generated with TRIS/EDTA (Art. No. DE006).

Applications:	IHC(C,P), WB

Incubation time:	60 min at RT or 18 hr at 2-8°C

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

SwissProt: P05090 (APOD_HUMAN)

References:
1. West R.B., Harvell J., Linn SC., Liu CL, Prapong W., Hernandez-Boussard T., Montgomery K., Nielsen TO., Rubin BP., Patel R., Goldblum JR., Brown PO., and van Rijn M. (2004) Apo D in soft tissue tumors: a novel marker for dermatofibrosarcoma protuberans. A

2. Lisovsky M., Hoang MP, Dresser KA., Kapur P., Bhawan J., Mahalingam M. (2008) Apolipoprotein D in CD34-positive and CD34-negative cutaneous neoplasms: a useful marker in differentiating superficial acral fibromyxoma from dermatofibrosarcoma protuberans. M

3. Stephen B. Hunter, Vijay Varma, Bahig Shehata, J.D.L. Nolen, Cynthia Cohen, Jeffrey J. Olson and Chin-Yih Ou (2005) Apolipoprotein D Expression in Primary Brain Tumors : Analysis by Quantitative RT-PCR in Formalin-fixed, Paraffin-embedded Tissue Journal o

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!