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Products> Enzymes

ALPI8G-2: Alkaline Phosphatase (AP)


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Price: 700 € / 2 ml

Enzyme

Protein concentration:app. 20-25 mg Protein/ml (batch dependent)
 
Origin:Cattle
 
Format:liquid
 
Quantity:2 ml
 
Concentration:app. 80.000 DEA U/ml
 
Presentation:Solution containing 50% glycerol, 5 mM Tris, 5 mM MgCl2 and 0,1 mM ZnCl3, pH 7
 
Specificity:Alkaline Phosphatase (EC 3.1.3.1)
 
Applications:Useful for immunohistochemistry and other immunological procedures. Conjugation to proteins (antibodies, avidin etc.) or non-covalent binding to anti-Alkaline Phosphatase for s. c.. APAAP system. Due to the high enzyme activity and turn over rate of the enzyme, very sensitive detection systems can be achieved. pH optimum at 9.6 - 9.8 (for IHC we recommend pH 8.2 to 8.5 dependent of the substrate composition).
 
Secondary reagents:The following commercially available substrates can be recommended for IHC: VECTOR Red (Art. No.. SK-5100), VECTOR Blue (Art.No. SK-5300) or Fast Red TM (Art. No. AP001).
 
Storage:2-8°C
 



Notes:
BIOLOGO products are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

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