ECM - TEN001: Tenascin C (TNC)

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TEN001: Tenascin C (TNC)

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Price: 310 € / 1 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	T2H5

Format:	liquid conc.

Quantity:	1 ml

Working concentration:	1:5-1:20

Presentation:	Cell culture medium with PBS, BSA and 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002.

Immunogen:	Purified Tenascin from mammary tumour cells

Specificity:	Human Tenascin, 210 and 300 kDa extracellular matrix protein

Species Specificity:	Human

General Information:	Tenascin is a high molecular ECM glycoprotein (MW app. 210-300 kDa), which contains hexamere repeat-rich single chains. It contains EGF-, Fibronectin III- and Fibronectin-similar structures. During organogenesis it is exprimed temporarily by many organs. During adulthood expression of the protein. It reappears only in regeneration, wound healing and in stroma of various tumours. Probably it has an anti-adhesive function, which plays a role during tumour speading. Tenascin is found in the central and peripheral nerve system, in smooth muscle, tendon and hyper-proliferative skin.

Special properties:	The antibody (T2H5) reacts with at least two forms of Tenascin (210 and 300 kD), an extracellular matrix glycoprotein. During embryonic development Tenascin appears to play a regulatory role in epithelial differentiation. In many mature tissues Tenascin disappears, but reappears in tissue regeneration and repair, as well as in neoplasia. Tenascin expression has been noted in a variety of epithelial neoplasms, including skin-, prostate-, breast- and colon cancer.

Positive control:	Colon carcinoma, abortive tissue, hyperproliferati

Pre-treatment:	Unmasking methods for paraffin sections are not established, but EDTA buffer (Ref. DE006) has been reported to be a suitable solution for high temperature unmasking.

Applications:	IHC(C), P not established

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Le Poole IC, van den Wijngaard RM, Westerhof W, Das PK. Tenascin is overexpressed in vitiligo lesional skin and inhibits melanocyte adhesion. Br J Dermatol. 1997 Aug;137(2):171-8.

2. Le Poole IC, van den Wijngaard RM, Westerhof W, Das PK. Presence of T cells and macrophages in inflammatory vitiligo skin parallels melanocyte disappearance. Am J Pathol. 1996 Apr;148(4):1219-28.

3. Rulo HF, van Vlijmen-Willems IM, Schalkwijk J, Verstraeten AA, van de Kerkhof PC. Normal human skin demonstrates marked site-variation of tenascin expression, not correlated to epidermal proliferation (Ki-67 binding).J Dermatol Sci. 1993 Feb;5(1):54-7.

4. Verstraeten AA, Mackie EJ, Hageman PC, Hilgers J, Schol DJ, De Jongh GJ, Schalkwijk J. Tenascin expression in basal cell carcinoma. Br J Dermatol. 1992 Dec;127(6):571-4.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!