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Products> ECM

Code N°


FI24941: Fibronectin, rat




Price: 470 € / 0.5 ml

Polyclonal Antibodies

Origin:Rabbit
 
Isotype:Ig
 
Clone:polyclonal
 
Format:purified, lyophilized
 
Quantity:0.5 ml
 
Reconstitution:Reconstitute with 0.5 ml distilled water, store aliquots at -20°C, for further dilution use appropriate antibody diluent e.g. Art. No. PU002
 
Concentration:app. 1 mg/ml
 
Working concentration:IFA 1:40, IHC(P) 1:200 - 1:600
 

Fibronectin on rat skin paraffin section. FI24941, DAB, hematoxylin


Presentation:0.5 ml affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added
 
Immunogen:Purified fibronectin from rat plasma
 
Specificity:Rat fibronectin 100%, rat collagen type I, III and IV <0.1%, rat elastin <0.1% (RIA at 1:5000 dilution)
 
Species Specificity:Rat
 
General Information:Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.
 
Special properties:This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.
 
Positive control:Rat skin
 
Pre-treatment:After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
 
Applications:IFA, IHC(P), ELISA, RIA
 
Incubation time:IHC(P) 60 min at RT or 2-8°C over night
 
Secondary reagents:Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:Biotin (Art. No. BA-1000)
 
Storage:2 - 8°C or aliquots at -20°C.
 

References:
See list of references in BIOLOGO ECM brochure.



Notes:
BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

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