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Products> BioPrime Antibodies

Code N°


PP040-1.0: p40, Delta-Np63




Price: 500 € / 1 ml

Rabbit monoclonal AB

Origin:Rabbit
 
Isotype:IgG
 
Clone:ZR8
 
Format:liquid conc.
 
Quantity:1 ml
 
Working concentration:1:50-1:100
 

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma stained with anti-p40 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells


Presentation:Affinity purified antibody in PBS, BSA, sodium azide (0.05%)**. Use antibody dilution buffer (e.g. Art. No. PU002) containing sufficient protein and preservative.
 
Immunogen:Synthetic polypeptides from N-terminal domaine of delta-Np63
 
Specificity:p40, delta-Np63
 
Species Specificity:Human
 
General Information:p63 consists of two major isoforms-TAp63 and Delta-Np63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation-competent ‘TA’ domain with homology to p53, which regulates the expression of the growth-inhibitory genes. In contrast, Delta-Np63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally-inactive ‘Delta-N’ domain, which antagonizes the activity of TAp63 and p53.
 
Special properties:The p40 (clone ZR8) antibody recognizes exclusively DNp63 but not TAp63. p40 is a squamous cell carcinoma specific antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung adenocarcinoma. p40 antibody can also be used as an alternative basal cell (myoepithelial cell) marker, which has similar sensitivity and specificity as p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma vs. invasive breast ductal carcinoma.
 
Positive control:Squamous Cell Carcinoma, Prostate, Skin
 
Pre-treatment:Pre-treatment of formaldehyde-fixed tissue with Tris-buffered EDTA solution pH 9 (Art.-No. DE006) or Unmasking Fluid G (Art.-No. DE007) 40 min at 100°C
 
Applications:IHC(P)
 
Secondary reagents:VECTASTAIN Elite ABC Rabbit IgG (PK-6101) or ImmPRESS Rabbit IgG (MP-7401) or other polymer kits detecting rabbit IgG.
 
Storage:2-8°C
 

References:
1. Guojun Wu, Shuji Nomoto, Mohammad Obaidul Hoque et al. (2003) ΔNp63α and TAp63α Regulate Transcription of Genes with Distinct Biological Functions in Cancer and Development Cancer Res May 15, 2003 63; 2351


2. Justin A Bishop, Julie Teruya-Feldstein, William H Westra, Giuseppe Pelosi, William D Travis and Natasha Rekhtman (2011) p40 (ΔNp63) is superior to p63 for the diagnosis of pulmonary squamous cell carcinoma. Modern Pathology (2012) 25, 405–415; doi:10.103


3. Verena Sailer, Carsten Stephan, Nicolas Wernert, Sven Perner, Klaus Jung, Manfred Dietel, Glen Kristiansen (2013) Comparison of p40 (ΔNp63) and p63 expression in prostate tissues – which one is the superior diagnostic marker for basal cells? Histopathology Volume 63, Issue 1, pages 50–56




Notes:
* BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.


** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!


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