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PIN203-1.0: PIN-Cocktail 2B - P504S (AMACR) / p63 / HMW Cytokeratin

Price: 920 € / 1 ml

Monoclonal Antibodies

Isotype:Rabbit IgG, Mouse IgG1
Clone:13H4; EPR5701; 34ßE12
Format:liquid, concentrated
Quantity:1 ml
Working concentration:Dilution 1:25-1:50
Presentation:Antibody cocktail in PBS pH 7.3, containing BSA and sodium azide* as preservative
Immunogen:1. Recombinant human AMACR (P504S) protein, whole sequence; 2. Recombinant human p63 protein aa 1-203; 3. Cytokeratin extract of human stratum corneum
Specificity:AMACR, p63, and HMW cytokeratin
Species Specificity:Human
General Information:P504S (AMACR, Alpha-methylacyl-CoA racemase) is an essential enzyme in the b-oxidation of branched-chain fatty acids. High expression of AMACR protein is found in prostate adenocarcinoma but not in benign prostate tissue by immunohistochemical staining in paraffin-embedded tissue. The expression of AMACR is also detected in prostate premalignant lesions, such as prostate intraepithelial neoplasia (PIN). The p63 protein, a homologue of the tumor-suppressor p53, is highly expressed in the nuclei of basal or progenitor layer of many epithelial tissues. p63 is detected in prostate basal cells in normal prostate glands and PIN. Other markers for basal cells are high molecular cytokeratins (CK 1, 5, 10 and 14), which can be labeled with HMW CK clone 34ßE12. In prostate adenocarcinoma basal cells disappear and staining with both p63 and HMW CK fails. Thus the combination of p63 and HMW CK is a useful tool as differential markers for benign prostate glands and adenocarcinoma (negative marker).
Special properties:The combination of AMACR (P504S), p63, and HMW CK may be extremely useful for diagnosing PIN and small focus adenocarcinoma, especially in difficult cases and cases with limited tissues. The combination of p63 and HMW CK antibodies provides a save method for the detection of basal cells in prostate tissues.
Positive control:Prostate Intraepithelial Neoplasia
Pre-treatment:TEC buffer (Tris/EDTA/Citrate) pH 8 in a pressure cooker at 100°C 20-40 minutes.
Applications:IHC, paraffin sections
Incubation time:30-60 min
Secondary reagents:Anti-Rabbit/anti-Mouse IgG double labeling system e.g. BIOLOGO's Universal staining kit DAB (Art. No. DA005) or AEC (Art. No. AE005) or other multi-link systems.

1. Jiang Z, Woda BA, Rock KL et al. P504S: a new molecular marker for the detection of prostate carcinoma. Am J Surg Pathol 2001; 25:1397-1404.

2. Weinstein MH, Signoretti S, Loda M: Diagnostic utility of immunohistochemical staining for p63, a sensitive marker of prostatic basal cells. Mod Pathol. 2002 Dec;15(12):1302-8.

3. Luo J, Shan Zha, Wesley R, et al. Alpha methylacyl-CoA racemase, a new molecular marker for prostate cancer. Cancer Res 2002; 62:2220-2226.

4. Browne TJ, Hirsch MS, Brodsky G, Welch WR, Loda MF, Rubin MA. Prospective evaluation of AMACR (P504S) and basal cell markers in the assessment of routine prostate needle biopsy specimens. Hum Pathol. 2004 Dec;35(12):1462-8.

5. Jiang Z, Li C, Fischer A, Dresser K, Woda BA. Using an AMACR (P504S)/34betaE12/p63 cocktail for the detection of small focal prostate carcinoma in needle biopsy specimens. Am J Clin Pathol. 2005 Feb;123(2):231-6.

P504S is a patented rabbit monoclonal antibody of CORIXA Co., USA, licensed and sublicensed to ZETA Co, USA and BIOLOGO, EUROPE.
BIOLOGO products are for in vitro research use only. They are not intended for clinical diagnostic use and must not be applied to living animals or humans.

* The preservative sodium azide has been added in very small quantities. Nevertheless, it may be harmful to health and the reagent should be used only by trained personnel. For waste disposal please refer to national regulations.

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