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Products> BioPrime Antibodies

Code N°


PIN001-0.5: PIN-Cocktail 1 - P504S (AMACR) + p63




Price: 380 € / 0.5 ml

Monoclonal Antibodies

Origin:Rabbit / Mouse
 
Isotype:Rabbit IgG, Mouse IgG2a
 
Clone:13H4 + 4A4
 
Format:Antibody cocktail
 
Quantity:0.5 ml
 
Working concentration:1:25-1:50 depending on unmasking method and secondary reagents
 

Prostate carcinoma, cytoplasmatic staining, basal cells with nuclear staining of benign glands


Presentation:Antibody cocktail in PBS pH 7.3, containing BSA and sodium azide** 0.09% as preservative
 
Immunogen:Human AMACR polypeptide + a recombinant protein derived from aa 1-205 of human p63
 
Specificity:Human AMACR (P504S) protein, Alpha-Methylacyl CoA Racemase and human p63 nuclear protein
 
Species Specificity:Human
 
General Information:AMACR is an essential enzyme in the beta-oxidation of branched-chain fatty acids. High expression of AMACR protein is found in prostate adenocarcinoma but not in benign prostate tissue by immunohistochemical staining in paraffin-embedded tissue. The expression of AMACR is also detected in prostate premalignant lesions, such as prostate intraepithelial neoplasia (PIN). The p63 protein, a homologue of the tumor-suppressor p53, is highly expressed in the basal or progenitor layer of many epithelial tissues. P63 is detected in prostate basal cells in normal prostate glands and PIN. However, it is negative in prostate adenocarcinoma. Thus p63 is useful as a differential marker for benign prostate glands and adenocarcinoma (negative marker). The combination of AMACR and p63 may be extremely useful for diagnosing PIN and small focus adenocarcinoma, especially in difficult cases and cases with limited tissues. This antibody cocktail may eliminate the need for 34ßE12. AMACR stains cytoplasm in prostate adenocarcinoma and PIN while p63 stains basal cell nuclei in PIN and benign prostate glands.
 
Special properties:The combination of AMACR and p63 may be extremely useful for diagnosing PIN and small focus adenocarcinoma, especially in difficult cases and cases with limited tissues. This antibody cocktail may eliminate the need for 34bE12. AMACR stains cytoplasm in prostate adenocarcinoma and PIN while p63 stains basal cell nuclei in PIN and benign prostate glands.
 
Positive control:Prostate intraepithelial neoplasia
 
Pre-treatment:TEC buffer (Tris/EDTA/Citrate) pH 8 in a pressure cooker at 100°C 20-40 minutes
 
Applications:IHC(C, P)
 
Staining procedure:Use formalin-fixed and paraffin-embedded sections; Retrieval conditions: 0.1 M citrate buffer (pH 6.0) in a steamer for 20 minutes; Use universal anti-mouse and anti-rabbit secondary antibody only; Working dilution: 1:40; Detection methods: ABC or EnVision Plus kits.
 
Storage:2-8°C
 
 

References:
1. Jiang Z, Woda BA, Rock KL et al. P504S: a new molecular marker for the detection of prostate carcinoma. Am J Surg Pathol 2001; 25:1397-1404.

2. Weinstein MH, Signoretti S, Loda M: Diagnostic utility of immunohistochemical staining for p63, a sensitive marker of prostatic basal cells. Mod Pathol. 2002 Dec;15(12):1302-8.

3. Luo J, Shan Zha, Wesley R, et al. Alpha methylacyl-CoA racemase, a new molecular marker for prostate cancer. Cancer Res 2002; 62:2220-2226.



Notes:
* PIN001 is sold for in vitro research purpose only, not for diagnostic or therapeutic use.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!


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