BioPrime Antibodies - P504S-G: AMACR (P504S) monoclonal antibody

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P504S-G: AMACR (P504S) monoclonal antibody

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Price: 120 € / 5 ml RTU

Monoclonal Antibodies

Origin:	Rabbit

Isotype:	IgG

Clone:	13H4

Format:	liquid, ready to use

Quantity:	5 ml RTU

Working concentration:	ready to use

PCa Gleason 3

PCa Gleason 4

PCa Gleason 5

Presentation:	Antibody solution in PBS, BSA, sodium azide 0.09%

Immunogen:	Recombinant P504S protein, complete sequence

Specificity:	Human P504S protein, alpha-Methylacyl CoA Racemase

Species Specificity:	Human

General Information:	Alpha-Methylacyl CoA Racemase ( AMACR) has originally been isolated and characterised from rat liver ( Schmitz et al., 1994, later also from human liver ( Schmitz et al., 1995). It is a peroxisomal and mitochondrial enzyme, specifically involved in beta-oxidation of branched fatty acids and fatty acid metabolites. Interestingly this enzyme by cDNA-subtraction and microarray screening has also been detected in prostate carcinoma ( Jiang et al., 2001). cDNA of human AMACR consists 1621 bases encoding a 382 AA protein.

Special properties:	The monoclonal Rabbit antibody directed against AMACR (P504S) shows predominant binding to prostate carcinoma tissue and only very faint or no binding to healthy benign prostate or benign prostate hyperplasia. Formaldehyde fixed and paraffin embedded tissue was investigated by Jiang et al. Among a total of 207 tissue samples all 137 carcinoma samples were P504S-positive, independent of Gleason-Score (V-VIII) (Jiang et al., 2001). Thus it may be possible to distinguish tumour epithelial cells from normal epithelial prostate tissue with help of this antibody. The expression of P504S in prostate is inversely related to that of high molecular weight cytokeratins as detected by clone 34ßE12. The majority (82,5%) of atypical adenomatous hyperplasia of prostate (n=40) reacted negative with this antibody, (82,5%), focal staining was observed in 10% and diffuse positive staining in 7,5% of cases (Yang et al., 2002). AMACR will also be found in neoplasm of other organs, e.g. colorectal carcinoma, mamma carcinoma, and renal cell carcinoma (Zhou et al., 2002).

Positive control:	Prostate carcinoma

Pre-treatment:	In formaldehyde-fixed tissue unmasking of antigen by heat pre-treatment is required. We recommend the application of our unmasking fluid G on glycol basis ( Art. No. DE007) 7-15 min in pressure cooker or 2 x 5 min micro wave oven. Citrate buffer is also suitable, but cell morphology usually is better with the other unmasking methods.

Applications:	The antibody is suitable for immunocytochemisty on cryostat and paraffin material. Western blotting has been described in literature, but has not been tested by BIOLOGO.

Incubation time:	60 min at room temperature (or 2-8°C over nig

Secondary reagents:	Any secondary system containing anti-rabbit Ig may be used. Best results have been generated with DAKO Envision Plus, VENTANA automation and VECTASTAIN ABC Systems ( Art.-No. PK-6101 or AK-5001) in combination with a suitable substrate.

Storage:	2-8°C

References:
1. Babcook J.S., Leslie K.B., Olsen O.A., et al. (1996) A novel strategy for generating monoclonal antibodies from single isolated lymphocytes producing antibodies of defined specificities. Proc. Natl. Acad. Sci. USA 93; 7843-7848.

2. Xu J., Stolk J.A., Zhang X., et al. (2000) Identificatin of differentially expressed genes in human prostate cancer using subtraction and microarray. Cancer Res. 60; 1677-1682.

3. Ferdinandusse S., Denis S., IJ1st, et al. (2000) Subcellular localization and physiological role of alpha-methylacyl-coA racemase. J. Lipid Res. 41; 1890-1896.

4. Jiang Z., Woda B.A., Rock K.L., Xu Y., Savas L., et al. (2001) P504S a new molecular marker for the detection of prostate carcinoma. Am. J. Surg. Pathol. 25(11); 1397-1404.

5. Yang X.J., Wu Ch.-L., Woda B.A., Dresser K., et al. (2002) Expression of alpha-methylacyl-CoA racemase (P504S) in atypical adenomatous hyperplasia of the prostate. Am. J. Surg. Pathol. 26(7); 921-925.

Notes:
** The preservative sodium azide has been added in very small quantities. Nevertheless it may be harmful to health and the reagent should be used only by trained personnel. For waste disposal please refer to national regulations.

BIOLOGO's antibodies may be used as research reagents for in vitro investigation only. They are not intended for clinical diagnostic use.