BioPrime Antibodies - CK5514: Cytokeratin 5 /14

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CK5514: Cytokeratin 5 /14

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Price: 270 € / 5 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1 + IgG3

Clone:	RCK 103 + LL002

Format:	ready to use

Quantity:	5 ml

Working concentration:	neat

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002 if required.

Immunogen:	Isolated cytokeratins from human epithelial cells for CK5 plus C-terminal 15-AA peptide of cytokeratin 14 conjugated to thyroglobulin

Specificity:	Human cytokeratin 5 and cytokeratin 14

Species Specificity:	Human

General Information:	Cytokeratins are a group of water insoluble filament proteins, which are constituents of the cytoskeleton of epidermal cells and other epithelial cells. By gel electrophoretic analysis up to now 20 different cytokeratins have been characterized. They have been divided into basic and acid subfamilies and may also be distinguished by their molecular weights and their tissue distribution. The most frequently used nomenclature has been published by R. Moll et al. (1982).

Special properties:	Antibody RCK103 reacts with cytokeratin 5 and neurofilaments. It shows a broad spectrum of reactivity and is exprimed by the majority of epithelial cells. The antibody may be used for the differentiation of carcinoma from non-epithelial tumours like lymphoma and sarcoma. The antibody is specially useful for the detection of cervical intraepithelial neoplasia (CIN). It binds as well to ectocervical as to endocervical cells and reserve cells. In prostate tissue CK5 may be used as a marker of basal cells (5). Additionally the antibody is of great help for the differential diagnosis of mesothelioma. Cytokeratin 14 (CK14) belongs to the acid subfamily of CKs (Type I) and is characteristic of stratifying epithelia (trachea, bronchi, cervix, epidermis, sweat glands). The antibody to CK14 is helpful to distinguish between stratifying epithelia and simple epithelia, which don't express this type of cytokeratins. Tumours of squamous cell origin will be stained with this antibody.

Positive control:	Skin, Cervix, Prostate

Pre-treatment:	Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid G (Art. No. DE007)

Applications:	C, P

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff.

2. Smedts F., Ramaekers F., Robben H., et al. (1990) Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J. Pathol. 136,3; 657-668.

3. Harnden P. and Southgate J. (1997) Cytokeratin 14 as a marker of squamous differentiation in transitional cell carcinomas. J Clin Pathol. 50(12):1032-1033.

4. Broekaert D. Leigh I.M., Laue E.B., Van Muijen G.N.P., Ramackers F.C.S., De Bersaques J. and P. Couke (1993) An immunohistochemical and histochemical study of cytokeratin, involucrin and tranglutaminase in seborrhoeic keratosis. Arch dermatol. Res. 285; 482-490.

5. Lakhani S.R., O'Hare M.J., Monaghan P., Winehouse J., Gazet J.C., Sloane J.P. (1995) Malignant myoepithelioma (myoepithelial carcinoma) of the breast: a detailed cytokeratin study. J. Clin. Pathol. 48(2):164-167.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!