BioPrime Antibodies - CK502: Cytokeratin, pan (AE1/AE3)

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CK502: Cytokeratin, pan (AE1/AE3)

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Price: 120 € / 5 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	AE1/AE3

Format:	ready to use

Quantity:	5 ml

Working concentration:	neat

Cytokeratin in Epidermis detected with CK502 (AE1/AE3) Universal Staining Kit Art. No. DA005, counterstain diluted Hematoxylin

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for app. 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Immunogen:	Human epithelial keratins

Specificity:	Basic cytokeratins (52-67 kD) and acid cytokeratins (40, 48, 50, 50', 56,5 kD) CK 1-8, 10, 14-16 and 19 according to the nomenclature by Moll (1982)

Species Specificity:	Human, chicken, cattle, rabbit, mouse, rat

General Information:	Cytokeratins are a group of water soluble filament proteins, which are components of the cytoskeleton of the epidermis and other epithelial cells. Gelelectrophoretic investigation revealed 20 different Cytokeratins, characterised by different molecular sizes and pI-values. They can be subdivided into basic and acid subfamilies. The nomenclature according to Moll et al. (1982) is most commonly used.

Special properties:	Pan-Cytokeratin (AE1/AE3) contains two different clones, which label the majority of normal epithelia and carcinoma.

Positive control:	Appendix

Pre-treatment:	Paraffin sections may be pre-treated with 1 % protease solution (Unmasking fluid P, Art.-No. DE110) for 15 min at RT, improvement of staining may be achieved with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000).

Applications:	IHC(P)

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff.

2. Sun T.-T. Tseng S.C.G., Huang A.J.W., Cooper D., Lynch M.H., Weiss R., Eichner R., and Schermer (1985) Monoclonal antibody studies of keratin expression: A review. In: Intermediate Filaments , Wang E. et al. eds. N.Y. Acad. Sci. 455, pp 307 ff.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!