BioPrime Antibodies - CD68-5: CD68 (Macrophages)

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CD68-5: CD68 (Macrophages)

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Price: 170 € / 5 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG3, k

Clone:	PG-M1

Format:	ready to use

Quantity:	5 ml

Working concentration:	neat

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Immunogen:	Gaucher cells

Specificity:	CD68 transmembran glycoprotein MW 110 kDa, localised in macrophages

Species Specificity:	Human

General Information:	CD68 is a transmembrane antigen, which is localised mainly in lysosomes. It belongs to the family of LAMP glycoproteins, which are involved in lysosomal transport and endocytosis respectively. The exact function of the CD68 molecule is not known.

Special properties:	The antibody PG-M1 is well suited for the detection of tissue macrophages including Kupffer cells and spleen macrophages. Among a number of CD68 antibodies PG-M1 is one of the most specific ones, since it stains neither myeloid precursor cells, nor granulocytes or dendritic cells. In acute myeloid leukaemia (AML) types M4 and M5 will be stained, while M1 - M3, M6 and M7 remain unstained.

Positive control:	Tonsil, spleen

Pre-treatment:	Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid C, E or G (Art. No. DE000, DE006, and DE007). Unmasking with Pronase (Art. No. DE110) or Proteinase K is also suitable.

Applications:	C, P

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Fukuda M. (1991) Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficing. J. Biol. Chem. 266; 21327-21330.

2. Holness C.I. and Simmons D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 81; 1607-1613.

3. Falini B. Flenghi L., Pileri S., Gambacorta M., Bigerna B., Durkop H., et al. (1993) PG-M1: a new monoclonal antibody directed against a fixative resistant epitope on the macrophage-restricted form of the CD68 molecule. Am. J. Pathol. 142; 1359-1372.

4. Falini B. Flenghi L., Pileri S., Stein H., D

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!