BioPrime Antibodies - CD5-1-1: CD5

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CD5-1-1: CD5

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Price: 350 € / 1 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	4C7

Format:	liquid conc.

Quantity:	1 ml

Working concentration:	1:10 - 1:25

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Immunogen:	Recombinant extracellular domaine of human CD5 molecule

Specificity:	Human CD5 molecule

Species Specificity:	Human

General Information:	CD5 is a lymphocyte surface molecule with a molecular weight of 67 kDa. It is exprimed by 95% of thymocytes and app. 72% of peripheral blood lymphocytes. In lymph nodes the antigen is detected in the T-cell region mainly. CD5 is over-expressed in leukaemia and T-cell lymphoma and may also be detected in a subpopulation of activated B-cells.

Special properties:	The antibody to CD5 is used for the classification of lymphoid neoplasm. CD5 is exprimed by the majority of T-cell lymphoma and is also found on B-cells in some cases of chronic lymphatic leukaemia and centrocytic lymphoma. Normally it is not found in other forms of lymphoid neoplasm e.g. follicular lymphoma, Hairy Cell Lymphoma and Large Cell Lymphoma.

Positive control:	Tonsil

Pre-treatment:	Paraffin sections are pre-treated with 1 mM EDTA (pH 8.0) in an autoclave (2 min under full pressure, cooling 15-20 min.)

Applications:	C, P, F

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Jones N.H., Clabby M.L., Dialynas D.P. et al. (1986) Isolation of complementary DNA clones encoding the human lymphocyte glycoprotein T1/Leu-1. Nature 323: 346-349.

2. Kasaian M.T., Ikematsu H. and Casali P. (1991) CD5+ B-Lymphocytes (43250). Proceedings of the Society for Experimental Biology and Medicine 197; 226-241.

3. Chan J.K.C., Banks P.M., Cleary M.L. (1994) A proposal for classification of lymphoid neoplasms (by the international lymphoma study group). Histopathology 25; 517-536.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!