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Products> BioPrime Antibodies

Code N°


CD3-1: CD3e, T cells






Monoclonal Antibodies

Origin:Mouse
 
Isotype:IgG1
 
Clone:F2.2.38
 
Format:conc. liquid
 
Quantity:1 ml
 
Working concentration:1:10
 
Presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
 
Immunogen:Purified CD3(epsilon, gamma, delta)/ CD3(omega)
 
Specificity:CD3(epsilon), cytoplasmic C-terminal
 
Species Specificity:Human
 
General Information:Lymphocytes may be characterized by CD (cluster of differentiation) molecules on the cell surface. The CD3 molecule was assigned as a CD marker on the 1. International Workshop on Human Leucocyte Differentiation Antigens (Paris 1982). The CD3 complex consists of 5 different glycoprotein chains and is characteristic for thymocytes and mature T-lymphocytes. Additionally CD3e is present on Purkinje-cells of the cerebellum.
 
Special properties:The antibody against CD3-epsilon stains as well medullary as cortical parts of the thymus and peripheral lymphatic tissues. The antigen can be located on most T-cell lymphoma. Exceptions to that are often large-cell anaplastic lymphoma (CD30-positive). B-cell lymphoma and non-lymphoidal tumours usually are unreactive.
 
Positive control:Lymph node, tonsil
 
Pre-treatment:Pre-treatment of formaldehyde-fixed material should be treated with Unmasking Solution E, TRIS-buffered EDTA pH 9 (Art. No. DE006) at 96-100°C
 
Applications:IHC(P)
 
Incubation time:60 min at RT
 
Secondary reagents:We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
 
Storage:2-8°C
 

References:
1. Garson J.A., Beverley P.C.L., Coakham H.B., Harker E.I. (1982) Monoclonal antibodies against human T lymphocytes label Purkinje neurons of many species. Nature 298; 375-377.

2. Campana D., Thompson J.S., Amlot P., Janossy G. (1987) The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. J. Immunol. 138; 648-655.

3. Mason D.Y., Cordell J., Brown M., et al. (1989) Detection of cells in paraffin wax embedded tissue using antibodies against a peptide sequence from the CD3 antigen. J. Clin. Pathol. 42; 1194-1200.

4. Anderson C., Rezuke W.N., Kosciol C.M., Pastuszak W.T., Cartun R.W. (1991) Identification of T-cell lymphomas in paraffin-embedded tissues using polyclonal anti-CD3 antibody: comparison with frozen section immunophenotyping and genotypic analysis. Modern Pathology 4; 358-362.

5. Alibaud L., Llobera R., Al Saati T., March M., Delsol G., and B. Rubin (2000) A new monoclonal anti-CD3epsilon antibody reactive on paraffin sections. J. Histochem Cytochem 48(12); 1609-1616.



Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

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