BioPrime Antibodies - CD20-1 (BC100): B Cells, CD20 (L26)

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CD20-1 (BC100): B Cells, CD20 (L26)

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Price: 270 € / 1 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG2a

Clone:	L26

Format:	liquid conc.

Quantity:	1 ml

Working concentration:	1:20 to 1:50

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Immunogen:	B-Cells from human Tonsils

Specificity:	Human CD20cy on peripheral and lymphoidal B-cells, germ center of lymph nodes.

Species Specificity:	Human

General Information:	CD20 is a non-glycosylated membran protein with a molecular mass of 33 kDa, which is typical for resting B cells. B cell activation leadrs to higher molecular isoforms of the protein, which will also be detected by the antibody L26. The antigen is absent from plasma cells (CD138+).

Special properties:	This antibody reacts extremly well with B cell lymphoma, in rare cases also T cell lymphoma may be stained. In Non-Hodgkin Lymphoma Reed Sternberg Cells will be stained.

Positive control:	Lymph nodes, Tonsil

Pre-treatment:	Improvement of staining with unmasking fluid G (Art. No. DE007) or unmasking fluid C (Art. No. DE000), frozen sections and cell smears should be fixed with ice cold acetone.

Applications:	C, P, FACS

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Zhou l.-Z. and Tedder T. (1993) CD20 Workshop panel report. In Schlossman S.F. et al. Eds. Leucocyte Typing V. White cell differentiatiation antigens. Oxford University Press, Oxford, N.Y., Tokyo; pp 511-514.

2. Takami T., Qi C., Yamada T., et al. (1989) B20.3. Reactivity and Specificity of L26 (pan-B-cell mAb) on 322 cases of fresh and paraffin-embedded lymphoproliferative diseases. In Kapp W. et al. eds. Leucocyte Typing IV. White Cell Diferentiation Antigens. Oxford University Press, Oxford, N.Y., Tokyo; pp 134-136.

3. Mason D.Y., Comans-Bitter WM., Cordell J.L., Verhoeven M.A.J. and van Dongen J.J.M. (1990) Antibody L26 recognizes an intracellular epitope of the B-cell-associated CD20 antigen. Am. J. Pathol. 136; 1215-1222.

Notes:
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.