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Products> BioPrime Antibodies

Code N°


CD15-1: CD15, human




Price: 250 € / 1 ml

Monoclonal Antibodies

Origin:Mouse
 
Isotype:IgM
 
Clone:MMA/LeuM1
 
Format:liquid conc.
 
Quantity:1 ml
 
Concentration:50 µg/ml
 
Working concentration:1:15-1:25
 
Presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
 
Immunogen:U937 histiocytic cell line
 
Specificity:Human CD15 (3-fucosyl-N-acetyllactosamine)
 
Species Specificity:Human
 
General Information:CD15 or X-Hapten plays a role in mediating phagocytosis, bacterial activity and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree monocytes. It is also present on Reed-Sternberg cells and on some epithelia.
 
Special properties:The antibody against CD15 CD15 is a helpful tool for the identification of Reed-Sternberg-Cells in Hodgkin's disease. Occasionally CD15 is exprimed also in large cell B- and T-cell lymphoma.
 
Positive control:Reed-Sternberg cells in Hodgkin's disease
 
Pre-treatment:Formaldehyde-fixed material should be treated with Unmasking Solution C, citrate buffered solution pH 6 (Art. No. DE000) at 96-100°C followed by short term treatment with Pepsin (0.1%)
 
Applications:IHC(P), FACS
 
Incubation time:60 min at RT
 
Secondary reagents:As a secondary reagent we recommend the use of a suitable anti-Mouse IgM conjugate e.g. biotinylated anti-Mouse IgM µ-chain specific (Art.-No. BA-2020).
 
Storage:2-8°C
 

References:
1. Skubitz K. et al. In: White Cell Differentiation Antigens. New York / Oxford University Press 1989; 800-805.



Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

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