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Products> BioPrime Antibodies

Code N°


CD11702-0.5: CD117 (SCFR, c-kit Oncoprotein), human




Price: 350 € / 0.5 ml

Monoclonal Antibodies

Origin:Rabbit
 
Isotype:IgG
 
Clone:YR145
 
Format:liquid conc.
 
Quantity:0.5 ml
 
Concentration:S/N
 
Working concentration:1:50-1:250
 
Presentation:Cell culture supernatant containing sodium azide 0.09%.
 
Immunogen:A synthetic peptide from C-terminus of human CD117 / c-kit protein
 
Specificity:Human CD117, c-kit Onkoprotein, SCFR (Stem Cell Factor Receptor)145 kDa protein in WB.
 
Species Specificity:Human, dog
 
General Information:CD117 encodes a transmembrane receptor with tyrosine kinase activity. This factor is closely related to the PDGF-R family. CD117 plays an important role during haematopoiesis, gametogenesis and melanogenesis. In normal tissues it is found in melanocytes, breast epithelium, glial cells and others. In tumours it is found in SCLC, Mamma carcinomas, melanomas, glioblastomas and in certain myelogenous leukaemias.
 
Special properties:The antibody to CD117 is suitable for the detection of CD117 in stem cells and for the characterization of certain Leukaemias (AML+, CML+, ALL almost neg.) It has been found to be a good marker of gastrointestinal stroma tumours (GIST; see Miettinen and Lasota, 2003) and has been applied for the definition of these tumours of mesenchymal origin.
 
Positive control:Skin, bone marrow, GIST
 
Pre-treatment:We recommend high temperature unmasking with Unmasking Fluid E (Tris-buffered EDTA solution pH 8.2, Art. No. DE006), which is advantageous to citrate buffer pre-treatment.
 
Applications:IHC (C,P)
 
Incubation time:60 min at RT
 
Secondary reagents:We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6101) or ABC AP (Art. No. AK-5001) systems are applicable.
 
Storage:2-8°C
 

References:
1. Natali P.G., Nicotra M.R., Winkler A.B., et al. (1992) Progression of human cutaneous melanoma is associated with loss of expression of c-kit proto-oncogene receptor. Int. J. Cancer 52; 197-201.

2. Matsuda R., Takahashi T., Nakamura S., et al. (1993) Expression of the c-kit protein in human solid tumors and in corresponding fetal and adult normal tissues. Am. J. Pathol. 142(1); 339-346.

3. Izquierdo M.A., Van der Valk P., Van Ark-Otte J. et al. (1995) Differential expression of the c-kit proto-oncogene in germ cell tumors. J. Pathol. 177; 253-258.

4. Miettinen M., and Lasota J. (2003) Gastrointestinal stromal tumors (GISTs): definition, occurence, pathology, differential diagnosis and molecular genetics. Pol J. Pathol. 54(1); 3-24.



Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

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