BioPrime Antibodies - CD117-1: CD117 (SCFR, c-kit Oncoprotein)

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CD117-1: CD117 (SCFR, c-kit Oncoprotein)

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Price: 410 € / 1 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	T595

Format:	liquid conc.

Quantity:	1 ml

Working concentration:	1:10

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Immunogen:	Recombinant protein sequence of the N-terminal extracellular C2-Domain of the human CD117 molecule

Specificity:	Human CD117, c-kit Onkoprotein, SCFR (Stem Cell Factor Receptor)

Species Specificity:	human

General Information:	C-kit Proto-Oncogen encodes a transmembrane receptor with tyrosine kinase activity. This factor is closely related to the PDGF-R family. CD117 plays an important role during haematopoiesis, gametogenesis and melanogenesis. In normal tissues it is found in melanocytes, breast epithelium, glial cells and others. In tumours it is found in SCLC, Mamma carcinomas, melanomas, glioblastomas and in certain myelogenous leukaemias.

Special properties:	The antibody to CD117 is suitable for the detection of CD117 in stem cells and for the characterization of certain Leukaemias (AML+, CML+, ALL almost neg.) Recently it has been found to be a good marker of gastrointestinal stroma tumours (GIST; see Miettinen and Lasota, 2003) and has been applied for the definition of these tumours of mesenchymal origin

Positive control:	Skin, bone marrow

Pre-treatment:	We recommend high temperature unmasking with Unmasking Fluid E (Tris-buffered EDTA solution pH 8.2, Art. No. DE006), which is advantageous to citrate buffer pre-treatment.

Applications:	IHC (C,P)

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C or -20°C

References:
1. Natali P.G., Nicotra M.R., Winkler A.B., et al. (1992) Progression of human cutaneous melanoma is associated with loss of expression of c-kit proto-oncogene receptor. Int. J. Cancer 52; 197-201.

2. Matsuda R., Takahashi T., Nakamura S., et al. (1993) Expression of the c-kit protein in human solid tumors and in corresponding fetal and adult normal tissues. Am. J. Pathol. 142(1); 339-346.

3. Izquierdo M.A., Van der Valk P., Van Ark-Otte J. et al. (1995) Differential expression of the c-kit proto-oncogene in germ cell tumors. J. Pathol. 177; 253-258.

4. Miettinen M., and Lasota J. (2003) Gastrointestinal stromal tumors (GISTs): definition, occurence, pathology, differential diagnosis and molecular genetics. Pol J. Pathol. 54(1); 3-24.

Notes:
*BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!