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Products> BioPrime Antibodies

Code N°


CD10-1: CD10 (CALLA)




Price: 440 € / 1 ml

Monoclonal Antibodies

Origin:Mouse
 
Isotype:IgG1
 
Clone:56C6
 
Format:liquid conc.
 
Quantity:1 ml
 
Working concentration:Dilution 1:10 -1:20
 
Presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
 
Immunogen:Recombinant human CD10
 
Specificity:CD10, human cell surface membrane glycoprotein (100 kDa)
 
Species Specificity:human, cross-reacting with rat
 
General Information:CD10 is an integral membrane protein with a 25 AA cytoplasmatic domain. The extracellular domain contains 12 cysteines and 6 positions for N-glycosylation. CD10 also called CALLA (common acute lymphoblastic leukaemia antigen) is exprimed by a number of different cell types including lymphoid progenitor cells, B cell line ALL and neutrophils. Germinal centre B cells and some leukaemia and non-lymphoid tissues like renal epithelia exhibit this glycoprotein, too.
 
Special properties:The antibody against CD10 is mainly used for phenotyping of leukaemia, in which it is a marker of a subset of ALL cells. It is used for differentiation of acute lymphoblastic leukaemia (ALL) from acute myeloic leukaemia (AML).
 
Positive control:Tonsil
 
Pre-treatment:Formaldehyde fixed tissue should be pre-treated for antigen retrieval in a high temperature buffer system e.g. citrate buffer pH 6.0 (art. no. DE000) or in TRIS-buffered EDTA solution (art. no. DE006)
 
Applications:The antibody may be used on cryostat or paraffin sections. Optimum conditions should be elaborated in each individual laboratory depending on fixation, secondary reagents and unmasking reagents and time.
 
Secondary reagents:We recommend a sensitive anti-mouse IgG conjugate as a secondary system e.g. horse-anti mouse IgG biotinylated (art. no. BA-2000) in combination with streptavidin : HRPO (art. no. SA-5004) and DAB substrate (art. no. DA002)
 
Storage:2-8°C
 

References:
1. H. Zola (1995) B2 CD10 Workshop Panel Report. In: Schlossman S.F. et al.eds. Leucocyte Typing V. White cell differentiation antigens. Oxford University Press, Oxford, N.Y., Tokyo pp. 505-507.

2. Kaufmann O, Flath B, Spath-Schwalbe E, Possinger K, Dietel M. Immunohistochemical detection of CD10 with monoclonal antibody 56C6 on paraffin sections. Am J Clin Pathol. 1999 Jan;111(1):117-22.

3. Chu P, Arber DA. Paraffin-section detection of CD10 in 505 nonhematopoietic neoplasms. Frequent expression in renal cell carcinoma and endometrial stromal sarcoma. Am J Clin Pathol. 2000 Mar;113(3):374-82.

4. Chu PG, Chang KL, Weiss LM, Arber DA. Immunohistochemical detection of CD10 in paraffin sections of hematopoietic neoplasms: a comparison with flow cytometry detection in 56 cases. Appl Immunohistochem Mol Morphol. 2000 Dec;8(4):257-62.

5. Ordi J, Romagosa C, Tavassoli FA, Nogales F, Palacin A, Condom E, Torne A, Cardesa A. CD10 expression in epithelial tissues and tumors of the gynecologic tract: a useful marker in the diagnosis of mesonephric, trophoblastic, and clear cell tumors. Am J Surg Pathol. 2003 Feb;27(2):178-86.



Notes:
* CD10-1 is designed for in vitro research use only. It must not be used in clinical diagnosis.

** Sodium azide is potentially hazardous to health. It should be used only by trained personal and in accordance with local rules.

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