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Products> BioPrime Antibodies

Code N°


CA150: E-Cadherin (Uvomorulin)




Price: 400 € / 1 ml

Monoclonal Antibodies

Origin:Mouse
 
Isotype:IgG1
 
Clone:SPM471
 
Format:liquid conc.
 
Quantity:1 ml
 
Concentration:50 µg/ml
 
Working concentration:1:10 - 1:50
 
Presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002.
 
Immunogen:Human Uvomorulin (E-Cadherin) Epitop aa 600-707
 
Specificity:E-Cadherin 120 kDa
 
Species Specificity:Human
 
General Information:E-Cadherin (Uvomorulin, ARC-1) is a Ca2+-dependent trans-membrane protein, which plays an important role for the cell connection in epithelia. Ligands for E-Cadherin are catenins, which bind to actin filaments of the cyto-skeleton. The cell to cell adhesion during embryo genesis is dependent of the interrelation of these three components. Reduced expression of E-Cadherin in carcinoma has been discussed as a factor influencing tumour invasion and bad prognosis of cancer.
 
Special properties:The antibody to E-cadherin is well suited to detect this glycoprotein in different types of epithelia. The localization is limited to the lateral cell membrane.
 
Positive control:Small intestine, appendix, corneal epithelium
 
Pre-treatment:Pre-treatment with Unmasking Fluid H (Art. No. DE008) at 96-100° C improves the staining results remarkably. For cryostat material acetone fixation (ice cold!) is recommended.
 
Applications:C, P
 
Incubation time:60 min at RT
 
Secondary reagents:We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
 
Storage:2-8°C
 

References:
1. Frixen U.H., et al. (1991) E-Cadherin-mediated cell-cell adhesion prevents invasivness of human carcinoma cells. J. Cell Biol. 113 ff.

2. Umbas R. Schalken J.A., Aalders T.W., Carter B.S., Karthaus H.F.M., Schaafsma H.E., Debruyne F.M.J., and Isaacs W.B. (1992) Expression of the cellular adhesion molecule E-Cadherin is reduced or absent in high-grade prostate cancer. Cancer Res. 52; 5104-5109.

3. Darai E., Scoazec J.Y., Walker-Combrouze F., Mlika-Cabanne N., Feldmann G., Madelenat P., and F. Potet (1997) Expression of cadherins in benign, borderline, and malignant ovarian epithelial tumors: a clinicopathologic study of 60 cases. Hum. Pathol. 28(8); 922-928.

4. Ruijter E., van de Kaa C., Aalders T. Ruiter D., Miller G., Debruyne F., and Schalken J. (1998) Heterogeneous expression of E-Cadherin and p53 in prostate cancer: clinical implications. BIOMED-II markers for prostate cancer study group. Mod. Pathol. 11(3); 276-281.

5. Stoemmer P and Bayer I. Invasion front phenomena in prostatic cancer. Virchow's Archiv(No.2 August 2007) page 123



Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

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