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Products> BioPrime Antibodies

Code N°


CA125-1: CA 125






Monoclonal Antibodies

Origin:Mouse
 
Isotype:IgG1
 
Clone:OV185.1
 
Format:Culture supernatant
 
Quantity:1 ml
 
Working concentration:1:20 - 1:100
 
Presentation:1 ml antibody in tissue culture supernatant containing sodium azide (0.09%). The volume is sufficient for 100-500 immunohistochemical stainings (100 µl working dilution/application). For dilution use appropriate antibody diluent e.g. Art. No. PU002.
 
Immunogen:Partially purified mucin fraction of an ovarian carcinoma
 
Specificity:Mucin-like glycoprotein >200 kDa containing CA 125-Epitop
 
Species Specificity:Human
 
General Information:Malign ovarian tumours account for app. 25% of all genital tumours in women. Among those tumours about 90% are of epithelial origin. More than 75% of all ovarian carcinoma result in increased levels of the cancer antigen CA 125 in serum.
 
Special properties:Cancer Antigen 125 is a glycoprotein of > 200 kDa, which is mainly found in ovarian carcinoma. The antibody OV185.1 reacts with the majority of ovarian carcinoma of mucinous, serous, endometroid and clear cell type. The antigen, however is not restricted to malign tumours.
 
Positive control:Mucinous adenocarcinoma of the ovary
 
Pre-treatment:In Formaldehyde-fixed tissue antigen unmasking at 96-100°C is required (e.g. Unmasking Fluid C Art. No. DE000 or Unmasking Fluid G Art. No. DE007).
 
Applications:The antibody may be used in immunohistochemical investigation as well on cryostat section as on paraffin embedded material. The optimal working dilution is dependent of the secondary reagents and should be titrated by every individual laboratory. Good result have been found at 1:20 dilution using anti-mouse IgG-HRPO-conjugate and at 1:100 using VECTASTAIN Elite ABC.
 
Secondary reagents:As a secondary system in immunohistochemistry we suggest the use of our Universal Staining Kits DAB and AEC (Art. No. DA005 and AE005) or the Mouse IgG Staining Kit (Art. No. DA007).
 
Storage:2-8°C or aliquots at -20°C
 

References:
1. Alogoz T., et al. (1994) What is normal CA 125 level? Gynecologic Oncology 53: 93-97.

2. Macri C., et al (1994) Highly elevated CA 125 and tubo-ovarian abscess mimicking ovarian carcinoma. Gynecol. Obstetric Invest. 37; 143-144.

3. Ordonez N.G. (1998) Role of immunohistochemistry in distinguishing epithelial peritoneal mesotheliomas from peritoneal and ovarian serous carcinomas. Am. Surg. Pathol. 22(10); 1203-1214.

4. Langendijk J.H., Mullink H., Van Diest P.J., Meijer G.A. and Meijer C.J. (1998) Tracing the origin of adenocarcinomas with unknown primary using immunocytochemistry: differential diagnosis between colonic and ovarian carcinomas as primary sites. Hum. Pathol.29(5); 491-497.



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