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Products> BioPrime Antibodies

Code N°


CA120: Calretinin




Price: 270 € / 1 ml

Polyclonal Antibodies

Origin:Rabbit
 
Isotype:Ig
 
Clone:polyclonal
 
Format:liquid conc.
 
Quantity:1 ml
 
Working concentration:1:40 - 1:50
 
Presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
 
Immunogen:Recombinant human Calretinin
 
Specificity:Calretinin
 
Species Specificity:Human, monkey, mouse, rat
 
General Information:Calretinin is a Calcium-binding protein related to Calmodulin and Calbindin. It is found in groups of neurons of the brain cortex, in the retina and in the spinal cord (1). More recent studies show that it is a suitable marker for mesothelioma of the lung (2, 3, 4, 5).
 
Special properties:The antibody is suitable for the detection of Calretinin in neurons and may be used as an aid in differential diagnosis of pleura-mesothelioma. This marker is frequently expressed in epitheloid mesothelioma, while occasionally it turns positive in sarcomatous mesothelioma and carcinoma. It was found to be negative in sarcoma (2,3) and in 90% of adenocarcinoma.
 
Positive control:Cerebral cortex, pleural mesothelioma
 
Pre-treatment:Improvement of staining with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000)
 
Applications:IHC (C, P)
 
Incubation time:60 min at RT
 
Secondary reagents:We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005), for higher sensitivities VECTASTAIN Elite ABC (Art. No. PK-6101) or ABC AP (Art. No. AK-5001) systems are applicable.
 
Storage:2-8°C
 

References:
1. Schwaller B., Buchwald P., Blümcke I., Celio M.R., and Hunziker W. (1994) Charakterization of a polyclonal antiserum against the purified human recombinant calcium binding protein calretinin. Cell Calcium 14; 639-648.


2. Gotzos V., Vogt P., and Celio M.R. (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192; 137-147.

3. Doglioni C., Tos A.P., Laurino L., Iuzzolino P., Chiarelli C., Celio M.R., and Viale G. (1996) Calretinin: a novel immunohistochemical marker for mesothelioma. Am. J. Surg. Pathol. 20; 1037-1046.

4. Wiethege T., Philips S. and Müller K.-M. (1997) Calretinin in Mesotheliomen und pulmonalen Adenokarzinomen. Ein differentialdiagnostischer Marker. Atemw. Lungenkrkh. 23(7); 410-411.

5. Müller K.-M. (1997) Mesotheliome, Pathologie / Pathogenese / Mesotheliomregister Pneumologie 51; 335-344.



Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

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