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Products> BioPrime Antibodies

Code N°


BR100: BrdU, Bromodesoxyuridine






Monoclonal Antibodies

Origin:Mouse
 
Isotype:IgG1
 
Clone:IIB5
 
Format:liquid conc.
 
Quantity:1 ml
 
Working concentration:1:5 - 1:20
 
Presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
 
Immunogen:Bromodesoxyuridine BSA-conjugate
 
Specificity:Bromodesoxyuridine and Iododesoxyuridine in denatured singlestranded DNA
 
Species Specificity:not species specific
 
General Information:Bromodesoxyuridine (BrdU) is an artificial thymidine analogue which can be taken up by proliferating cells and incorporated into complementary DNA. So called puls labelling allows the detection of the S phase of mitosis. In cell culture usually incubation of cells with BrdU at an end concentration of 10 µM for 10 min usually is sufficient for labelling of cells in vitro. In-vivo experiments usually require parenteral application of app. 5 mg/kg BrdU. Ex-vivo labelling of small pieces of tissue may be performed in culture medium containing 10 µM BrdU.
 
Special properties:The antibody IIB5 may be used for the detection of incorporated BrdU or IrdU in cell nuclei of proliferating cells.
 
Pre-treatment:Formaldehyde-fixed paraffin sections are treted with Pepsin and must undergo acid denaturation in front of antibody application. Cryostat material is fixed with alcohol and denatured with acid to generate single stranded DNA.
 
Applications:The antibody may be used on paraffin or cryostat material (2,3). Additionally it is suitable for the detection of BrdU by flow cytometry (1).
 
Incubation time:60 min at RT
 
Secondary reagents:We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
 
Storage:2-8°C
 

References:
1. Schutte B., et al. (1987) Studies with anti-Bromodeoxyuridine antibodies I. An improved method for the immunocytochemical detection of BrdU labelled nuclei using flow cytometry. Cytometry 8; 372-376.

2. Schutte B., et al. (1987) Studies with anti-Bromodeoxyuridine antibodies II. Simultaneous immunohistochemical detection of antigen expression and DNA synthesis by in vivo labelling of mouse intestinal mucosa. J. Histochem. Cytochem. 35(3); 371-374.

3. Schutte B., et al. (1987) Effect of tissue fixation on anti-Bromodeoxyuridine immunohistochemistry. J. Histochem. Cytochem. 35(11); 1343-1345.



Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

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