Home|About BioLogo|Ordering|Contact|Help|News|GTC|Sitemap |Deutsch
   register
line
logo

Antibodies – Recombinant Proteins – Staining Reagents


for Biology and Medicine – Consultancy – Development – Production - Distribution
line
Search:
line
Products:
line
AGE
line
BioPrime Antibodies
line
BioPrime Staining Reagents
line
BioPrime Accessories
line
ECM
line
ELISA
line
Enzymes
line
Research
line
Proteins and Peptides
line
VECTOR Reagents
line
  Print data sheet   warenkorb_large (1K) add to basket

Products> BioPrime Antibodies

Code N°


BC115: B Cells, MB3 (act. T Cells), Histiocytes






Monoclonal Antibodies

Origin:Mouse
 
Isotype:IgG2b
 
Clone:MB3
 
Format:liquid conc.
 
Quantity:1 ml
 
Working concentration:1:5-1:20
 
Presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
 
Immunogen:Human lymphocytes
 
Specificity:31 kD protein fraction on human B-lymphocytes
 
Species Specificity:Human
 
Special properties:The antibody MB3 is an excellent marker of all B cells. It is especially well suited for the immunocytochemical and histochemical detection of B cells in formaldehyde fixed tissue (also Bouin's fixative). It is used for the immunophenotyping of lymphoma and multiple myeloma. It does not react with normal T cells but only with activated ones. In non-haematopoietic tissue MB3 especially reacts with histiocytes, glandular epithelia, smooth muscle, endothelial cells and peripheral nerve tissue.
 
Positive control:Lymph nodes, Tonsil
 
Pre-treatment:No protease pre-treatment required, blocking of endogenous peroxidases is recommended.
 
Applications:C, P
 
Incubation time:60 min at RT
 
Secondary reagents:We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or VECTOR ImmPress micropolymer kits Mouse IgG (Art.-No. MP-7402) are applicable.
 
Storage:2-8°C
 

References:
1. Poppema S., Hollema H., Visser L, and H. Vos (1987) Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin embedded tissue sections. Am. J. Pathol. 127,3: 418-429

2. Fukuda T, Ohnishi Y, Hasegawa T, Kakihara T, Usuda H. (1992) Distribution of antigens detected with MB1, MB2 and MB3 on non-hematopoietic human organs and various tumors. Acta Pathol Jpn 1992 May;42(5):339-346.

3. Fukuda T, Ohnishi Y, Hasegawa T, Kakihara T, Usuda H. (1992) Distribution of antigens detected with MB1, MB2 and MB3 on non-hematopoietic human organs and various tumors. Acta Pathol Jpn 1992 May;42(5):339-346.



Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

 Print page


» Product list

BioLogo | Steindamm 1 G-H | 24119 Kronshagen | Germany | Phone +49 (0)431.5842 801 | Fax +49 (0)431.5842 803 | info@biologo.de