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Products> BioPrime Antibodies

Code N°


BC110: B Cells, MB2






Monoclonal Antibodies

Origin:Mouse
 
Isotype:IgG1
 
Clone:MB2
 
Format:liquid conc.
 
Quantity:1 ml
 
Working concentration:1:10-1:100
 
Presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
 
Immunogen:Human lymphocytes
 
Specificity:28 kDa protein fraction of human B-lymphocytes
 
Species Specificity:Human
 
Special properties:The antibody MB2 is an excellent marker of all B cells. It is especially well suited for the immunocytochemical and histochemical detection of B cells in formaldehyde fixed tissue (also Bouin's fixative). MB2 usually doesn't react with T cells. It is used for the immunophenotyping of lymphoma and multiple myeloma. In non-haematopoietic tissue MB2 especially reacts with glandular epithelia, smooth muscle, endothelial cells and peripheral nerve tissue.
 
Positive control:Lymph nodes, Tonsil
 
Pre-treatment:No protease pre-treatment required, blocking of endogenous peroxidases is recommended.
 
Applications:C, P
 
Incubation time:60 min at RT
 
Secondary reagents:We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or VECTOR ImmPress micropolymer kits Mouse IgG (Art.-No. MP-7402) are applicable.
 
Storage:2-8°C
 

References:
1. Poppema S., Hollema H., Visser L, and H. Vos (1987) Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin embedded tissue sections. Am. J. Pathol. 127,3: 418-429

2. Hall PA, D'Ardenne AJ, Butler MG, Habeshaw JR, Stansfeld AG (1987) New marker of B lymphocytes, MB2: comparison with other lymphocyte subset markers active in conventionally processed tissue sections. J. Clin. Pathol.40(2):151-156.

3. Dehou MF, Schots R, Lacor P, Arras N, Verhavert P, Kloppel G, van Camp B. (1990) Diagnostic and prognostic value of the MB2 monoclonal antibody in paraffin-embedded bone marrow sections of patients with multiple myeloma and monoclonal gammopathy of undetermined significance. Am J Clin Pathol 94(3):287-291.

4. Schmid C, Pan L, Diss T, Isaacson PG.(1991) Expression of B-cell antigens by Hodgkin's and Reed-Sternberg cells. : Am J Pathol 139(4):701-707.

5. Fukuda T, Ohnishi Y, Hasegawa T, Kakihara T, Usuda H. (1992) Distribution of antigens detected with MB1, MB2 and MB3 on non-hematopoietic human organs and various tumors. Acta Pathol Jpn 1992 May;42(5):339-346.



Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

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