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Products> BioPrime Antibodies

Code N°


AM110: Amyloid A (REU-86.2)






Monoclonal Antibodies

Origin:Mouse
 
Isotype:IgG2a
 
Clone:REU-86.2
 
Format:liquid concentrate
 
Quantity:1 ml
 
Working concentration:1:10
 
Presentation:Cell culture supernatant containing 0.09% sodium azide
 
Immunogen:Human Serum-Amyloid A (Apo-SAA)
 
Specificity:Human Amyloid Type AA
 
Species Specificity:Human
 
General Information:Amyloidosis describes a disease during which fibrillary proteins are secreted into the extracellular space of different organs. Histological stainings demonstrate eosinophilic deposits, which progressively displace surrounding parenchymatous tissues, resulting in the loss of organ function and eventually death. Chronic inflammation (type AA-amyloidosis) and plasma cell dyscrasia (type AL-amyloidosis) are the most frequent forms of amyloidosis occurring e.g. in kidney and rectal tissue. In neighbourhood of hemodialysis more frequently cases of amyloidosis of the B2M type have been reported.
 
Special properties:The antibody REU-86.2 detects amyloid type AA. It may be used for the detection of protein deposits (which are not easily detected by HE staining), and will help distinguishing from amyloid types AL and B2M. The antibody may also be used for screening purposes in kidney and rectal biopsies.
 
Positive control:Kidney with amyloid deposits
 
Pre-treatment:No protease pre-treatment required, but microwave treatment may intensify staining. We refer to BIOLOGO's Unmasking Fluid G (Art. No. DE007).
 
Applications:IHC (C, P)
 
Incubation time:60 min at RT
 
Secondary reagents:We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
 
Storage:2-8°C
 

References:
1. Francis R.J. (1982) Amyloid. In: Theory and practice of histological techniques. Bancroft J.D. and Stevens A. eds. pp 155-179.

2. Linke R.P. (1984) Monoclonal antibodies against amyloid fibril protein AA. J. Histochem. Cytochem. 32(3); 322-328.

3. Donini U., Casanova S., Zucchelli P., et al. (1989) Immunoelectron microscopic classification of amyloid in renal biopsies. J. Histochem. Cytochem. 37(7); 1101-1106.

4. Bouke P C Hazenberg, Pieter C Limburg, Johan Bijzet, Martin H van Rijswijk (1999) A quantitative method for detecting deposits of amyloid A protein in aspirated fat tissue of patients with arthritis Ann Rheum Dis 1999;58:96-102




Notes:
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

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