Home|About BioLogo|Ordering|Contact|Help|News|GTC|Sitemap |Deutsch
   register
line
logo

Antibodies – Recombinant Proteins – Staining Reagents


for Biology and Medicine – Consultancy – Development – Production - Distribution
line
Search:
line
Products:
line
AGE
line
BioPrime Antibodies
line
BioPrime Staining Reagents
line
BioPrime Accessories
line
ECM
line
ELISA
line
Enzymes
line
Research
line
Proteins and Peptides
line
VECTOR Reagents
line
  Print data sheet   warenkorb_large (1K) add to basket

Products> BioPrime Antibodies

Code N°


AA105: Alpha-Actin, sarcomeric




Price: 240 € / 1 ml

Monoclonal Antibodies

Origin:Mouse
 
Isotype:IgM
 
Clone:5C5
 
Format:liquid conc.
 
Quantity:1 ml
 
Working concentration:1:10 - 1:50
 
Presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution/test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
 
Immunogen:Purified rabbit skelet-muscle Actin
 
Specificity:Alpha-Actin sarcomeric (a-sr-1) specific for skelet- and heart-muscle
 
Species Specificity:Human, amphibia, bony fish, cattle, guinea pig, reptiles, rabbit, rat, sheep
 
General Information:Movement of cell organelles, cells, and organisms depends on the interaction of the cytoskeletal proteins actin and myosin. In birds and mammals up to now six different isoforms of actin have been identified using amino acid sequence analysis and electrophoresis. They are more or less specific for different organs resp. cell types. Four isoforms are known to occur in muscle cells, while two other forms are found in most other cell types.
 
Special properties:The antibody 5C5 which is directed against sarcomeric alpha-actin labels skeletal and heart muscle cells but not smooth muscle cells. In neoplasms it may be used as a marker of rhabdomyosarcoma.
 
Positive control:Skelet muscle
 
Pre-treatment:No protease pre-treatment necessary. Blocking of endogenous peroxidase and alkaline phosphatase recommended depending on the detection system in use.
 
Applications:C, P
 
Incubation time:60 min at RT
 
Secondary reagents:We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
 
Storage:2-8°C
 

References:
1. Skalli O, Gabbiani G, Babai F, Seemayer TA, Pizzolato G, Schurch W. Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. II. Rhabdomyosarcomas.
Am. J. Pathol., 130; 515ff (1988)

2. Babai F, Skalli O, Schurch W, Seemayer TA, Gabbiani G. Chemically induced rhabdomyosarcomas in rats. Ultrastructural, immunohistochemical, biochemical features and expression of alpha-actin isoforms.Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;55(5):263-77.

3. Schurch W, Skalli O, Seemayer TA, Gabbiani G. Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. I. Smooth muscle tumors. Am J Pathol. 1987 Jul;128(1):91-103

4. Hasegawa T., Hirose T., Kudo E., Abe J., and Hizawa K. (1990) Cytosteletal characteristics of myofibroblasts in benign and reactive fibroblastic lesions. Virch. Arch. (A) Pathol. Anat. 416; 375-382



Notes:
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

 Print page


» Product list

BioLogo | Steindamm 1 G-H | 24119 Kronshagen | Germany | Phone +49 (0)431.5842 801 | Fax +49 (0)431.5842 803 | info@biologo.de