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Products> BioPrime Antibodies

Code N°


AA100: Alpha-Actin, smooth muscle




Price: 240 € / 1 ml

Monoclonal Antibodies

Origin:Mouse
 
Isotype:IgG2a
 
Clone:1A4
 
Format:liquid concentrate
 
Quantity:1 ml
 
Working concentration:1:10 - 1:50
 
Presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution/test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
 
Immunogen:Synthetic N-terminal decapeptide of smooth muscle Alpha-Actin
 
Specificity:Alpha-Actin, smooth muscle
 
Species Specificity:Human and other vertebrates
 
General Information:Movement of cell organelles, cells, and organisms depends on the interaction of the cytoskeletal proteins actin and myosin. In birds and mammals up to now six different isoforms of actin have been identified using amino acid sequence analysis and electrophoresis. They are more or less specific for different organs resp. cell types. Four isoforms are known to occur in muscle cells, while two other forms are found in most other cell types.
 
Special properties:The antibody 1A4 detects alpha-actin of smooth muscle origin, thus it is suitable to distinguish smooth muscle cells from cells of sarcomeric origin. It may also be used for differentiation from fibroblast actin (F-actin).
 
Positive control:Intestine, Appendix
 
Pre-treatment:No protease pre-treatment necessary, but microwave treatment usually will intensify staining. We refer to BIOLOGO's Unmasking Fluid G (Art. No. DE007).
 
Applications:C, P, WB
 
Incubation time:60 min at RT
 
Secondary reagents:We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
 
Storage:2-8°C
 
 

References:
1. Skalli O, Ropraz P., Trzeciak A., Benzonana G., Gillessen D. Gabbiani G. (1986) A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J. Cell Biol. 103; 2787-2796

2. Skalli O., Schürch W., Seemayer T., Lagacé R., Montandon D., Pittet B., et al. (1989) Myofibroblasts from diverse pathologic settings are heterogenous in their content of actin isoforms and intermediate filament proteins. Lab Invest. 60; 275-285

3. Rønnov-Jessen L., Van Deurs B., Celis J.E., and Petersen O.W. (1990) Smooth muscle differentiation in cultured human breast gland stromal cells. Lab. Invest. 63; 532-543

4. Hasegawa T., Hirose T., Kudo E., Abe J., and Hizawa K. (1990) Cytosteletal characteristics of myofibroblasts in benign and reactive fibroblastic lesions. Virch. Arch. (A) Pathol. Anat. 416; 375-382



Notes:
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

BIOLOGO antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

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