Research - VIP001-050: VIP, Vasoactive Intestinal Peptide

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VIP001-050: VIP, Vasoactive Intestinal Peptide

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Price: 480 € / 50 µl

Polyclonal Antibodies

Origin:	Rabbit

Isotype:	Ig

Clone:	polyclonal

Format:	lyophil.

Quantity:	50 µl

Reconstitution:	Reconstitute with distilled water or PBS, mix only after 10 min, further dilution should be made with PBS or TBS containing 1% cristallin BSA and 0.09% sodium azide.

Working concentration:	1:1000 - 1:5000

Presentation:	Lyophilized original serum

Immunogen:	Purified porcine VIP (Mutt)

Specificity:	VIP, Vasoactive Intestinal Polypeptide, antibody blocking with small amounts of VIP, but not with Secretin, PHI, Glucagon, GIP and CCK

Species Specificity:	Human, cat, pig, rat, other vertebrates, also cartilaginous fish (Sciliorhinus stellaris).

General Information:	Vasoactive intestinal peptide (VIP) is a 28-amino-acid neuropeptide hormone. Its sequence is identical in man, ox, pig and rat. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumours secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhoea, hypokalemia and achlorhydria syndrome. VIP shows high sequence homology with peptide histidine-methionine (PHM) in humans and peptide histidine-serine (PHI) in pig or rat and is exprimed in the same precursor.

Positive control:	Small intestine, ileum

Pre-treatment:	Pre-treatment of paraffin material is not required, but enhanced staining may occur after application of high temperature unmasking (method not established).

Applications:	IHC(C, P)

Incubation time:	60 min at RT or 18 hr at 4 degrees C

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	-20'°C'

References:
1. Ekblad E., et al. (1984) VIP and PHI coexist with the NPY-like peptide in intramural neurons of the small intestine. Reg. Pept. 10; 47-55.

2. Tagliafierro G., et al. (1988) Distribution and ontogeny of VIP-like immuno-reactivity in the gastro-entero-pancreatic system of a cartilaginous fish Sciliorhinus stellaris. Cell Tissue Res. 253; 23-28.

3. Ekström J., et al. (1994) Developmental studies on VIP, SP and cGRP in salivary glands of postnatal rats. Acta Physiol. Scand. 151; 107-115.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.