Research - SPB001: Surfactant Protein B-Precursor (Pro-SP-B)

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SPB001: Surfactant Protein B-Precursor (Pro-SP-B)

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Price: 360 € / 1 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG2a

Clone:	SPB01 (1-2-A5-9)

Format:	liquid conc.

Quantity:	1 ml

Concentration:	50 µg/ml

Working concentration:	2-4 µg/ml

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Immunogen:	Recombinant Pro-Surfactant Protein B

Specificity:	Pro-Surfactant Protein B, no reactivity with mature SPB

Species Specificity:	Human, Rat, others not tested

General Information:	Lung surfactant is a complex of phospholipids and proteins which is secreted by type II alveolar cells. It is decreasing the surface tension in the airways and is essential for unobstructed ventilation. Up to now 4 different proteins have been isolated and have been defined as surfactant proteins A, B, C and D: SP-A and SP-D are collagenous carbohydrate binding proteins while SP-B and SP-C are non-collagenous proteins.

Special properties:	SP-B is exprimed in adenocarcinoma of the lung with acinar, papillary, brochioloalveolar and solid growth pattern. Squamous cell carcinoma and large cell carcinoma of the lung as well as non-pulmonary adenocarcinoma don't express SP-B. Pro-SP-B is glycosylated in the Golgi apparatus and undergoes carboxyterminal and aminoterminal proteolysis to form the mature SP-B.

Positive control:	Lung adenocarcinoma

Pre-treatment:	After formaldehyde fixation antigen unmasking at 96-100°C is recommended (use citrate buffer Art. No. DE000 or Unmasking Fluid G on glycol basis Art. No. DE007 respectively).

Applications:	IF, IHC(P)

Incubation time:	60 min at RT

Secondary reagents:	As secondary systems in immunohistochemistry we recommend the VECTASTAIN Elite ABC Mouse IgG system (Art. No. PK-6102) or VECTOR ImmPress Mouse IgG (Art. No. MP-7402) and BIOLOGO Universal Staining Kits DAB and AEC (Art. No. DA005 and AE005).

Storage:	2-8°C

References:
1. Zhou L., Lim L., Costa R.H., and Whitsett J.A. (1996) Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung. J. Histochem. And Cytochem. 44(10);1183-1193.

2. Khoor A. ; Whitsett J. A. ; Stahlman M. T. ; Olson S. J. ; Cagle P. T. (1999) Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma Human pathology 30(6);695-700.

3. Korimilli A., Gonzales L.W., and Guttentag S.H. (2000) Intracellular Localization of Processing Events in Human Surfactant Protein B Biosynthesis* J Biol Chem, 275(12); 8672-8679.

4. Kolla V., Gonzales L.W., Gonzales J., Wang P., Angampalli S., Feinstein S.I., and Ballard P. L. (2007) Thyroid Transcription Factor in Differentiating Type II Cells: Regulation, Isoforms, and Target Genes Am. J. Respir. Cell Mol. Biol.36(2): 213 - 225.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!