Research - NFK001: NF kappa B / p65

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NFK001: NF kappa B / p65

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Price: 380 € / 100 µg

Polyclonal Antibodies

Origin:	Rabbit

Isotype:	IgG

Clone:	polyclonal

Format:	liquid conc.

Quantity:	100 µg

Concentration:	200 µg/ml

Working concentration:	IHC (2 µg/ml), WB (2-5 µg/ml)

Presentation:	Affinity purified IgG fraction from rabbit serum in PBS containing 0.2% BSA and 0.09% NaN3

Immunogen:	Synthetic peptide from internal domain of NFkB p65

Specificity:	NFkB (65kDa)

Species Specificity:	Human, mouse, rat (other vertebrates expected, but not tested)

General Information:	NFkB is a heterodimer protein consisting of a 50kDa DNA-binding subunit and a 65kDa transactivation subunit (RelA). The heterodimer is present in cytoplasm in inactive form bound to its inhibitor IkB. In case of cellular stimulation IkB becomes phosphorylated and releases active NFkB for nuclear translocation.

Positive control:	Prostate, placenta

Pre-treatment:	Unmasking with citrate buffer pH 6 10-20 min at 100°C

Applications:	WB, IHC(P)

Incubation time:	IHC(P) 60 min at RT or 2-8°C over night

Secondary reagents:	Biotinylated anti-Rabbit IgG (Art. No. ZU101) in combination with Streptavidin:HRPO (Art. No. ZU054) or Streptavidin:AP (Art. No. ZU051) and a suitable substrate.

Storage:	2-8°C

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!