Research - MU103: Mucin 3

Products:

AGE

BioPrime Antibodies

BioPrime Staining Reagents

Proteins and Peptides

VECTOR Reagents

Products> Research

MU103: Mucin 3

Print page

place in shopping cart

Price: inquiry ! € / 1 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG2a

Clone:	M3.1

Format:	liquid conc.

Quantity:	1 ml

Working concentration:	1:10 - 1:20

Presentation:	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Immunogen:	Synthetic MUC-3 fragment 35 aa containing the tandem repeat sequence of MUC-3: C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS

Specificity:	Hochmolecular glycoprotein MUC-3 and epitope SITTTE, no cross-reactivity with HMFG, MUC-1 and MUC-2.

Species Specificity:	Human

General Information:	Mucins are a group of high molecular glycoproteins, which are produced and secreted by glandular epithelia. They are used for the protection of epithelia of the lung, stomach, intestine and the uro-genital tracts. Until now 7 different mucins have been described, which show a characteristic pattern of distribution in different organs and parts of the intestine respectively. This pattern usually changes during carcinogenesis.

Special properties:	The antibody M3.1 reacts with normal cells of the colon and with colon carcinoma. Glandular epithelia of the breast, ling and salivary glands are stained to a lesser extent.

Positive control:	Colon carcinoma and normal colon

Pre-treatment:	Acetone fixation of cryostat and fresh material

Applications:	ELISA, Western Blot, Immunohistochemistry (cryostat sections only)

Incubation time:	60 min at RT

Secondary reagents:	We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage:	2-8°C

References:
1. Kim Y.S., Gum J.R., Byrd J.C., Toribara N.W. (1991) The structure of human intestinal apomucins. Am. Rev. Resp. Disease 144; 10-14.

2. Ho S.B., Niehans G.A., Lyftogt C., Yan P.S., Cherwitz D.L., Gum E.T., Dahiya R., Kim Y.S. (1993) Heterogeneity of mucin gene expression in normal and neoplastic tissues. Cancer Res. 53(3); 641-651.

3. Sasaki M., Nakanuma Y., Terada T., Kim Y.S. (1995) Biliary epithelium expression of MUC1, MUC2, MUC3 and MUC5/6 apomucins during intrahepatic bile duct development and maturation. An immunohistochemical study. Am J. Pathol. 147(3); 574-579.

4. Weiss A.A., Babyatsky M.W., Ogata S., Chen A., Itzkowitz S.H. (1996) Expression of MUC2 and MUC3 mRNA in human normal, malignant and inflammatory intestinal tissues. J. Histochem. Cytochem 44; 1161-1166.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!