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Products> Research
HYD001: 25(OH)Vitamin D3-1-alpha-Hydroxylase
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place in shopping cart Price: 300 € / 100 µl
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| Presentation: | Sheep IgG fraction in glycine buffered NaCl solution pH 7.4; contains 0.1% sodium azide, 0.1% Epsilon-amino-n-caproic acid, 0.01% Benzamidine and 1 mM EDTA |
| Immunogen: | Synthetic peptid from the coding sequence of murine 25(OH)-Vitamin-D3-1-alpha-Hydroxylase (aa 266-289) |
| Specificity: | 25(OH)Vitamin D3-1-alpha-Hydroxylase, 56 kDa Protein in Western Blot |
| Species Specificity: | Mouse, human |
| General Information: | The seco-steroid hormone 1,25-Dihydroxyvitamin D3 plays a vital role in Calcium metabolism, in tissue differentiation and during the growth of bone. The biosynthesis of the hormone is catalysed by a mitochondrial P450-enzyme, 25-Hydroxy-Vitamin D3 1-alpha-Hydroxylase. Although the enzyme has been detected in different tissues, the main site of Vitamin D3 synthesis is the kidney. |
| Special properties: | The polyclonal antibody is well suited for the detection of 25(Hydroxy)Vitamin D3-1-alpha-Hydroxylase in tissues and tissue extracts e.g. in HKC-8 cell line from human proximal tubules (1). |
| Positive control: | Kidney |
| Applications: | Western blot and immunohistochemistry |
| Secondary reagents: | As a secondary reagent we recommend a biotinylated antibody directed against sheep IgG (e.g. VECTOR Art. No. BA-6000 or BA-6080 in combination with Vectastain ABC Standard Art. No. PK-6100) and a suitable peroxidase substrate (e.g. BIOLOGO DAB Art. No. DA002 or AEC Art. No. AE002). |
| Storage: | 2-8°C |
References:
1. Bland R, Walker EA, Hughes SV, Stewart PM, Hewison M. (1999) Constitutive expression of 25-hydroxyvitamin D3-1-alpha-hydroxylase in a transformed human proximal tubule cell line: Evidence for direct regulation of vitamin D metabolism by calcium. Endocrinology 140(5), 2027-2034
2. Zehnder D, Bland R, Walker EA, Bradwell AR, Howie AJ, Hewison M, Stewart PM. (1999) Expression of 25-Hydroxyvitamin D3-1-alpha-hydoxylase in human kidney. J. Am. Soc. Nephrol. 10; 2465-2473
3. Bland R, Markovic D, Hills CE, Hughes SV, Chan SL, Squires PE, Hewison M (2004) Expression of 25-hydroxyvitamin D3-1alpha-hydroxylase in pancreatic islets. J Steroid Biochem Mol Biol. 89-90(1-5):121-125.
4. Takeyama K, Kitanaka S, Sato T, Kobori M, Yanagisawa J, Kato S. (1997) 25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis. Science;277(5333):1827-30 (cloning of 1-alpha-hydroxylase)
5. Agic A, Xu H, Altgassen C, Noack F, Wolfler MM, Diedrich K, Friedrich M, Taylor RN, Hornung D. Relative expression of 1,25-dihydroxyvitamin D3 receptor, vitamin D 1 alpha-hydroxylase, vitamin D 24-hydroxylase, and vitamin D 25-hydroxylase in endometriosis and gynecologic cancers. Reprod Sci. 2007 Jul;14(5):486-97.
Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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