Research - HEP001-G: Hep Par-1 (Hepatocyte Specific Antigen)

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HEP001-G: Hep Par-1 (Hepatocyte Specific Antigen)

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Price: 210 € / 5 ml

Monoclonal Antibodies

Origin:	Mouse

Isotype:	IgG1

Clone:	OCH1E5

Format:	liquid conc.

Quantity:	5 ml

Working concentration:	neat

Presentation:	Purified mouse IgG1 in PBS, stabilized with BSA and NaN3

Immunogen:	Formalin-fixed, rejected liver transplant

Specificity:	Hepatocellular antigen of the mitochondrial fraction

Species Specificity:	Human

General Information:	Hep Par-1 (Hepatocyte Paraffin 1) antibody is a relatively specific marker of hepatocytes, which is a useful reagent in the differential diagnosis of liver tumours. It is specially well suited for the discrimination of hepatocellular clear-cell tumours from other clear-cell tumours and for the differentiation of hepatoblastoma (especially of the embryonal typs) from other small roundcellular tumours in childhood. Parallel investigation with tumor markers like Alpha-Fetoprotein (AFP) and Carcinoembrional Antigen (CEA) are recommended.

Special properties:	In normal tissue all hepatocytes are stained with this marker. There seams to be no zonal preference for the expression of the marker, however bile ducts and non-parenchymal tissues are unstained. Also other tissues like bladder, skin, lung and other tissues can occasionally show focal staining. Hepatocellular carcinoma show high rates of positivity (>97%), negative results usually are due to sclerosing tissue.

Positive control:	Liver

Pre-treatment:	Formaldehyd-fixed tissue should be pre-treated with Unmasking Fluid E (TRIS/EDTA pH. 9; Art. No. DE006), citrate buffer doesn't perform equally well.

Applications:	IHC (C,P)

Incubation time:	IHC(P) 60 min at RT or 2-8°C over night

Secondary reagents:	VECTASTAIN ImmPress™ mouse IgG (Art. No. MP-7402) in combination with a suitable peroxidase substrate. If biotinylated antibodies are preferred, please consider that hepatic tissue may contain high concentrations of endegenous biotin.

Storage:	2-8°C

References:
1. Zhen Fan, Matt van de Rijn, Kelli Montgomery and Robert V Rouse. Hep Par 1 Antibody Stain for the Differential Diagnosis of Hepatocellular Carcinoma: 676 Tumors Tested Using Tissue Microarrays and Conventional Tissue Sections Mod Pathol 2003;16(2):137-144.

2. T P Thamboo, A Wee Hep Par 1 expression in carcinoma of the cervix: implications for diagnosis and prognosis Journal of Clinical Pathology 2004;57:48-53.

3. Kanitakis J, Chouvet B, Claudy A, Scoazec JY. Immunoreactivity of hepatocyte paraffin 1 monoclonal antibody in cutaneous metastatic tumors. Am J Clin Pathol. 2004 Jul;122(1):85-9.

Notes:
*BIOLOGO's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!